Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (32): 6021-6026.doi: 10.3969/j.issn.1673-8225.2011.32.030

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Effects of assemble flavone of rhizoma drynariae on proliferation and differentiation of rabbit bone marrow mesenchymal stem cells

Liu Wei, Zhao Jin-min, Su Wei, Li Xiao-feng, Qin Yi-wu   

  1. Department of Orthopedic Trauma and Hand Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Received:2011-02-14 Revised:2011-04-11 Online:2011-08-06 Published:2011-08-06
  • Contact: Zhao Jin-min, Professor, Doctoral supervisor, Department of Orthopedic Trauma and Hand Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China zhaojinmin@126.com
  • About author:Liu Wei★, Studying for master’s degree, Department of Orthopedic Trauma and Hand Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China jxgxliuwei@163.com
  • Supported by:

    Key Program of Natural Science Foundation of Guangxi, No.0991014Z*

Abstract:

BACKGROUND: Rhizoma drynariae is commonly used in orthopedics and showing an accurate effect; however, it specific mechanisms are not very clear.
OBJECTIVE: To explore the influences of assemble flavone of rhizoma drynariae on proliferation and differentiation of rabbit bone marrow mesenchymal stem cells (BMSCs).
METHODS: The rabbit BMSCs were isolated from femur and tibia bone marrow by combination of gradient centrifugation and different adherent method, then proliferated and purified in vitro. The influence on the rabbit BMSCs proliferation caused by different concentration of assemble flavone of rhizoma drynariae was observed with methyl thiazdyl tetrazolium (MTT) assay; rabbit BMSCs were treated with assemble flavone of rhizoma drynariae inductor in vitro, then to observe the morphology by scanning electron microscope (SEM), identified by alkaline phosphatase staining, and the calcium node formation was detected by alizarin bordeaux staining and von Kossa staining.
RESULTS AND CONCLUSION: Rabbit BMSCs could be isolated and cultured by combination of gradient centrifugation and different adherent method. After co-culturing by assemble flavone of rhizoma in vitro, the results of MTT showed that the assemble flavone of rhizoma drynariae with 10-6 mmol/L had an obvious promotion on the rabbit BMSCs proliferation. After assemble flavone of rhizoma drynariae induction, osteoblast-like cell morphology and calcium node formation could be observed under SEM; after dyeing, cells were positive to alkaline phosphatase staining, alizarin bordeaux staining and von Kossa staining. Assemble flavone of rhizoma drynariaecan can accelerate the proliferation and differentiation of rabbit BMSCs.

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