Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (32): 6027-6031.doi: 10.3969/j.issn.1673-8225.2011.32.031

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Effects of high glucose and Astragalus Membranaceus on the number, proliferation, and differentiation of endothelial progenitor cells from human peripheral blood in vitro

Xu Han-song1, Lei Min-xiang2, Kong De-ming1, Xiang Hui3, Xie Xiao-yun4   

  1. 1Department of Endocrinology, the Second Affiliated Hospital of Guiyang College of TCM, Guiyang  550003, Guizhou Province, China
    2Department of Endocrinology, Xiangya Hospital of Central South University, Changsha  410008, Hunan Province, China
    3Department of Psychology, Guizhou Provincial People’s Hospital, Guiyang  550003, Guizhou Province, China
    4Department of Endocrinology, Third Xiangya Hospital of Central South University, Changsha  410008, Hunan Province, China
  • Received:2011-02-01 Revised:2011-03-14 Online:2011-08-06 Published:2011-08-06
  • About author:Xu Han-song☆, Doctor, Associate professor, Department of Endocrinology, the Second Affiliated Hospital of Guiyang College of TCM, Guiyang 550003, Guizhou Province, China xuhansong911@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30960491*; Guizhou Grovernor Funding for Excellent Education Professionals*

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Abstract:

BACKGROUND: The quantity and impaired function of endothelial progenitor cells (EPCs) is the important link of diabetic vascular complications. Astragalus Membranaceus (AMI) can protect endothelium of dysfunction which caused by various reasons and can effectively protect the function of vascular endothelium.
OBJECTIVE: To investigate the effects of high glucose and AMI on the number, proliferation, and differentiation of EPCs from human peripheral blood in vitro.
METHODS: EPCs were incubated with various concentrations of glucose for 24 hours and with 30 mmol/L glucose for different periods. Simultaneously, EPCs were pre-incubated with 20 g/L AMI for 24 hours and then all the cells were incubated with 30 mmol/L glucose for 24 hours.
RESULTS AND CONCLUSION: High glucose decreased the number of EPCs and abilities of EPCs proliferation and differentiation in a dose- and time-dependent manner (P < 0.05 or 0.01). Pre-incubated with AMI could increase EPCs quantity and improve EPCs proliferative and differentiative capacity (P < 0.05 or 0.01). AMI can increase the number and improve the capacity of proliferation and differentiation of EPCs cultured with high glucose.

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