Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (32): 6032-6035.doi: 10.3969/j.issn.1673-8225.2011.32.032

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Effects of Ginsenoside Rg1 on the expressions of Nanog, c-Myc, Oct, Klf4, Sox2 mRNA during the differentiation of bone marrow mesenchymal stem cells into pluripotent stem cells

Li Jia-wei 1, 2, Zhang Mi-xia 1, 2, Zhou Tao 1, 2, Torao Ishida2, Wang Xiu-yun 1, 2   

  1. 1Tianjin University of Traditional Chinese Medicine, Tianjin  300193, China
    2Institute of Traditional Chinese Medicine, Suzuka University of Medical Science, Suzuka 510-0293, Japan
  • Received:2011-02-26 Revised:2011-06-01 Online:2011-08-06 Published:2011-08-06
  • Contact: Wang Xiu-yun, Institute of Traditional Chinese Medicine, Suzuka University of Medical Science, Suzuka 510-0293, Japan; Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China wxyzhaq@126.com
  • About author:Li Jia-wei★, Master, Technician, Institute of Traditional Chinese Medicine, Suzuka University of Medical Science, Suzuka 510-0293, Japan; Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China lijiawei1981@163.com
  • Supported by:

    the “High-Tech Research Center” Project of Japan (MEXT.HAITEKU, 2006-2010. Project number: 05H018, 2005. 16 Japan Education Science High-Tech 978)*

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Abstract:

BACKGROUND: Somatic cells are reprogrammed to induced pluripotent stem cells (iPSCs), mainly through anti-retroviral program to transfer Oct-4, Sox2, c-Myc, Klf4 and other genes into somatic cells.
OBJECTIVE: To observe the effects of Ginsenoside Rg1 on Oct4, Sox2, c-Myc, Klf4, Nanog mRNA expression during differentiation of bone marrow mesenchymal stem cells (BMSCs).
METHODS: The BMSCs were cultured; 6.25 μmol/L and 12.5μmol/L Ginsenoside Rg1 of Chinese medicine extraction were added into culture medium, respectively. Oct4, Sox2, c-Myc, Klf4, Nanog mRNA expressions were detected in BMSCs.
RESULTS AND CONCLUSION: In the BMSCs cultured by 6.25 μmol/L Ginsenoside Rg1 for 30 days, the expressions of Nanog, c-Myc, Oct, Klf4, Sox2 mRNA of BMSCs were increased, and the expression of the gene Nanog and c-Myc had significantly difference compared with the control group. It demonstrated that Ginsenoside Rg1 could enhance the expressions of Nanog, c-Myc, which are crucial genes on converting the BMSCs to iPS. But Nanog positive iPS cells are very difficult to be discriminated from the embryonic stem cells in gene expression profile; therefore, we consider that Ginsenoside Rg1 might facilitate the differentiation from BMSCs to iPS.

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