Abstract:

BACKGROUND: Bing’s giant salamander has the effect to enhance the fracture healing, but the action mechanism and active constituent are still unclear.
OBJECTIVE: To investigate the effects of the serum of Bing’s giant salamander on the proliferation and osteogenic differentiation of rat bone marrow mesenchymal stem cells (rBMSCs) by different concentrations.
METHODS: Rats were given Bing’s giant salamander by high, moderate and low concentration, respectively, and their serum was obtained after 7 days. The control serum was obtained by giving equal volume physiological saline. rBMSCs were obtained from Wistar rats and screened by the adhesive method. The serum was supplemented into the culture medium of third passage rBMSCs by different concentration (2.5%, 5%, 7.5%, 10%). The proliferation of rBMSCs was analyzed by MTT reduction assay. The osteogenic differentiation markers including alkaline phosphatase (ALP) activity, calcium deposition, Collagen Ⅰ, osteocalcin and mineralized bone modulus were compared among the exposed groups and the control.
RESULTS AND CONCLUSION: The values of A570 in serum groups containing 5% and 7.5% Bing’s giant salamander were significantly higher than those of other concentrations and the control. 5% was stronger than 2.5% in significantly enhancing the osteogenic differentiation of rBMSCs, indicated by significantly improved ALP activity, calcium deposition, Collagen Ⅰ, osteocalcin and the number of mineralized bone nodules compared to the control and other groups (P < 0.01). The metabolites of Bing’s giant salamander after oral administration stimulate the proliferation and osteogenic differentiation of rBMSCs, indicating that they are effective to treat the patients with delayed fracture healing.