Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (32): 5945-5950.doi: 10.3969/j.issn.1673-8225.2011.32.013

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Remnant-like particles activate peroxisome proliferator-activated receptor gamma and induce adipogenic differentiation of human adipose mesenchymal stem cells

Ma Mei-fang1, Wen Tie2, Liu Ling1   

  1. 1Department of Cardiology, 2Department of Surgical Emergency, Second Xiangya Hospital, Central South University, Changsha  410011, Hunan Province, China
  • Received:2011-05-20 Revised:2011-06-12 Online:2011-08-06 Published:2011-08-06
  • Contact: Liu Ling, Associate professor, Master’s supervisor, Department of Cardiology, Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China feliuling@medmail.com.cn
  • About author:Ma Mei-fang★, Master, Physician, Department of Cardiology, Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China wish0818@163.com
  • Supported by:

    Special Project Funds for Atherosclerosis Research of Chinese Medical Association, No. 09010570212*; Outstanding Youth Foundation of Hunan Province, No. 10JJ1008*; the Project Fund from Research Center of Metabolic Syndrome, Second Xiangya Hospital, Central South University, No. DY-2008-01-01*

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Abstract:

BACKGROUND: Remnant-like particles (RLPs) can induce rat adipose-derived mesenchymal stem cells (ADSCs) to differentiate into mature adipocytes.
OBJECTIVE: To explore the effects of RLPs on the adipogenic differentiation of human ADSCs and on mRNA expressions of peroxisome proliferator-activated receptor γ (PPAR-γ) and adiponectin (APN).
METHODS: After stimulation by standard cocktail inducer, lipid droplets within adipocytes were identified by oil red O staining. Human RLPs were isolated by density gradient ultracentrifugation and immuno-affinity chromatography from postprandial plasma of hypertriglyceridemic patients at 4 hours after a high-fat meal. ADSCs were stimulated by 10 mg/L insulin and different concentrations of RLPs (0, 50, 100, 150, 200 mg/L). 
RESULTS AND CONCLUSION: After stimulation at 12 days, a small amount of lipid droplets was found in the control group or  50 mg/L RLPs stimulated ADSCs by oil red O staining. Lipid droplets increased gradually with the increase of RLPs concentrations from 100 to 200 mg/L and reached the peak in 200 mg/L RLPs stimulated ADSCs. The mRNA expressions of PPAR-γ and APN increased with the increase of RLPs concentrations from 50-200 mg/L (P < 0.05). APN mRNA expression in 200 mg/L RLPs group was significantly weaker than that in 150 mg/L RLPs group (P < 0.05), while it showed no significant difference when compared with that in 100 mg/L RLPs group. It indicates that RLPs induce adipogenic differentiation of human ADSCs through activating PPARγ, and up-regulate APN expression.

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