Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (32): 5951-5956.doi: 10.3969/j.issn.1673-8225.2011.32.014

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Differentiation potential of human Orbicularis oculi muscle-derived stem cells towards Schwann cells phenotype

Ding Wei-jin1,2, Zhang Wen-jun1,2, Sun Mei-qing1,2, Su Zhi-da2, Li Cui2, Liu An-tang1,2, Jiang Hua1,2   

  1. 1Plastic and Reconstructive Department of Changzheng Hospital, Second Military Medical University of Chinese PLA, Shanghai  200003, China; 2Neuroscience Research Center of Changzheng Hospital, Second Military Medical University, Shanghai  200433, China
  • Received:2011-04-23 Revised:2011-06-15 Online:2011-08-06 Published:2011-08-06
  • Contact: Jiang Hua, Ph.D., Professor, Chief physician, Plastic and Reconstructive Department of Changzheng Hospital, Second Military Medical University of Chinese PLA, Shanghai 200003, China; Neuroscience Research Center of Changzheng Hospital, Second Military Medical University, Shanghai 200433, China docdwj@163.com
  • About author:Ding Wei-jin☆, Doctor, Plastic and Reconstructive Department of Changzheng Hospital, Second Military Medical University of Chinese PLA, Shanghai 200003, China; Neuroscience Research Center of Changzheng Hospital, Second Military Medical University, Shanghai 200433, China dingweijin@yahoo.cn
  • Supported by:

    the Key Basic Research Project of Shanghai, No. 08JC1407100*

Abstract:

BACKGROUND: Muscle derived stem cells (MDSCs) can be isolated from human orbicularis oculi muscle and be differentiated to a Schwann cell phenotype which could eventually provide functional benefits for peripheral nerve repair.
OBJECTIVE: To induce the differentiation of MDSCs into Schwann cell phenotype.
METHODS: ①Under the support of microscope, we collected the discarded human Orbicularis oculi muscle resected in the upper eyelid blepharoplasty and isolate human-MDSCs within it with aid of tri-enzyme digestion and pre-plating technique, and then identify the cells by immunohistochemistry method. ②We isolated Schwann cells and identify the cells by immunohistochemistry method. Through half-harvest method, we would like to prepare conditioned medium from Schwann cell culture. ③We co-culture human-MDSCs with Schwann cell conditioned medium and the transdifferentiated cell morphology was investigated daily under microscope. The common used marker, S-100, GFAP and p75 were stained to identify Schwann cell phenotype with use of immunohistochemistry method.
RESULTS AND CONCLUSION: ①We collected human Orbicularis oculi muscle sample from three young female volunteer with their consensus. Human-MDSCs were isolated from Orbicularis oculi muscle and have their desmin positively stained and Sca-1 was positively expressed. ②Schwann cells were isolated and identified with S-100 positively stained at the rate of (97.4±0.7)%. ③The isolated human-MDSCs were successfully transdifferentiated into Schwann cell-like cells with positive expression of S100, GFAP and p75, which would serve as a unanimous evidence of Schwann cell phenotype. Human-MDSCs could be transdifferentiated into Schwann cell-like cells when co-cultured within Schwann cell conditioned medium, which would serve as an alternative candidate for commonly studied Schwann cells in tissue engineering nerve graft.

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