Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (32): 5901-5904.doi: 10.3969/j.issn.1673-8225.2011.32.003

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Immunoregulatory function of fetal bone marrow-derived mesenchymal stem cells on human Th17 cells

Guo Zhen-xing1, Zheng Cui-ling2, Chen Zhen-ping3, Dong Wen-chuan1, Yang Ren-chi4   

  1. 1Department of Hematology/Oncology, First Hospital of Tsinghua University, Beijing  100016, China
    2Department of Clinical Laboratory, Cancer Hospital, Chinese Academy of Medical Sciences, Beijing  100021, China
    3Hematology Center, Beijing Children’s Hospital, Beijing  100045, China
    4State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences, Tianjin  300020, China
  • Received:2011-02-28 Revised:2011-03-28 Online:2011-08-06 Published:2011-08-06
  • About author:Guo Zhen-xing☆, Doctor, Department of Hematology/ Oncology, First Hospital of Tsinghua University, Beijing 100016, China gzx2962@yahoo.com.cn
  • Supported by:

     the National Natural Science Foundation of China, No. 81000228*

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Abstract:

BACKGROUND: Many studies have demonstrated mesenchymal stem cell (MSC) can regulate the immune function and inhibit proliferation of T cell.
OBJECTIVE: To investigate the regulatory function of fetal bone marrow-derived mesenchymal stem cells (FBM-MSCs) on human Th17 cells.
METHODS: FBM-MSCs were cocultured with human peripheral blood mononuclear cells (PBMCs) or CD4+ T cells at 1:10 ratio from healthy donors for 4 days. Single culture of mononuclear cells or CD4+ T cells were as controls. The expression of interleukin-17 (IL-17) mRNA was detected by real-time PCR, IL-17 protein was assayed by enzyme-labeled immunosorbent assay (ELISA), and quantity of Th17 cells was observed by flow cytometry.
RESULTS AND CONCLUSION: The level of IL-17mRNA in FBM-MSC cocultured with PBMC (FBM-MSC/PBMC) group was significantly higher than that in PBMC cultured alone. Meanwhile, IL-17 expression in supernatant in FBM-MSC/PBMC/ CD4+ was significantly higher than that in PBMC and CD4+ T cells groups ( P < 0.05, P < 0.01). The number of FBM-MSCs/CD4+ T cells group was significantly more than CD4+ T cells group ( P < 0.01). However, FBM-MSCs did not express IL-17 protein. FBM-MSCs can promote the proliferation of Th17 cells.

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