Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (32): 5896-5900.doi: 10.3969/j.issn.1673-8225.2011.32.002

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Improvement of methods to isolate and culture human mesenchymal stem cells

Wang Cheng-yun1, Shi Lei2, Xia Chun2, Zheng Xin-peng2, Zhang Bing3, Lin Fei-tai3, Wang Shao-jie2   

  1. 1Fujian Medical University Postgraduate College, Fuzhou  350108, Fujian Province, China
    2Department of Joint Surgery, Zhongshan Hospital of Xiamen University, Xiamen  361004, Fujian Province, China
    3Xiamen University School of Medicine, Xiamen  361005, Fujian Province, China
  • Received:2011-02-28 Revised:2011-03-28 Online:2011-08-06 Published:2011-08-06
  • Contact: Xia Chun, Professor, Doctor, Chief physician, Master’s supervisor, Department of Joint Surgery, Zhongshan Hospital of Xiamen University, Xiamen 361004, Fujian Province, China Chunxia99@yahoo.com.cn
  • About author:Wang Cheng-yun★, Studying for master’s degree, Fujian Medical University Postgraduate College, Fuzhou 350108, Fujian Province, China hanson19830920@126.com
  • Supported by:

    Xiamen Technology Foundation Sub-project in 2009, No. 3502Z20094008*

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Abstract:

BACKGROUND: The methods to isolate mesenchymal stem cells (MSCs) can be classified to be several kinds. However, the method of acquisition in surgery is still less. The isolated MSCs from the patients who receive the replacement operation can offer some data which may reveal the causes or clues of some diseases.
OBJECTIVE: To isolate and culture MSCs with an improved technique of differential anchoring velocity and adherence, in order to identify MSCs.
METHODS: The bone marrow blood was collected in the femur marrow cavity duiring the hip joint replacement. MSCs were isolated by adherence method in the special medium and the mix medium was flushed 36-48 hours after the inoculation to isolate MSCs from the marrow mixture. In the long-time culture in vitro, the special medium was used to weed other cells out. The anchored cell was cultured and identified when the amount was large enough. 
RESULTS AND CONCLUSION: MSCs can be isolated from the bone marrow in long cavitas medullaris, and can be cultured into colonies by using adherence method. The morphological features and surface molecules are identified consistent with MSCs, and can obtain standard MSCs, so as to do the follow-up experiment.

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