Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (32): 5891-5895.doi: 10.3969/j.issn.1673-8225.2011.32.001

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Modalities of the first medium exchange during the culture of rat bone-marrow derived mesenchymal stem cells

Ni Ping1, Chen Zi-qian2, Peng De-xin2, Bai Wei2   

  1. 1Department of Medical Engineering, 2Center of Imaging, Fuzhou General Hospital of Nanjing Military Command of Chinese PLA, Fuzhou  350025, Fujian Province, China
  • Received:2011-02-12 Revised:2011-03-18 Online:2011-08-06 Published:2011-08-06
  • Contact: Chen Zi-qian, Chief physician, Professor, Doctoral supervisor, Center of Imaging, Fuzhou General Hospital of Nanjing Military Command of Chinese PLA, Fuzhou 350025, Fujian Province, China chenziqianfz@yahoo.con.cn
  • About author:Ni Ping★, Master, Senior engineer, Department of Medical Engineering, Fuzhou General Hospital of Nanjing Military Command of Chinese PLA, Fuzhou 350025, Fujian Province, China nping6@sohu.com
  • Supported by:

    the Social Development Project of Fujian Province, No. 2009J001187*; the Natural Science Foundation of Fujian Province, No. 2009Y0037*; Medical Innovation Project of Nanjing Military Command, No. 09Z029*

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Abstract:

BACKGROUND: The culture period, quantity and purity are crucial for stem cell transplantation, and it is essential to build an optimal modality of cell culture in vitro. 
OBJECTIVE: To study the influence of different modalities of the first medium exchange on the culture time and biological characteristics of rat bone marrow-derived mesenchymal stem cells.
METHODS: On condition of modality of the first medium exchange, six experimental groups were performed as follows: group A was half exchanged after 24 hours, group B was all exchanged after 24 hours, group C was half exchanged after 48 hours, group D was all exchanged after 48 hours, group E was half exchanged after 72 hours, group F was all exchanged after 72 hours. Then, all groups were all exchanged every 48 hours and were subcultured consecutively. The period and purity of each passage were measured.
RESULTS AND CONCLUSION: The total culture time was significant different (F=46.455, P < 0.001). Group D was the least while group B was the longest, and both of the two groups were significant different compared with others (P < 0.05). Group B earned the highest purity in all passages and group E the lowest, and both of the two groups were significant different compared with others (P < 0.05). Cells of passage 4 showed CD29, CD90 positive, induced to differentiate into osteoblasts and adipocytes. The modality of the first medium exchange had an influence on the culture period and purity of bone marrow-derived mesenchymal stem cells, and the modality of 48-hour half exchange was the best protocol.

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