Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (32): 5905-5908.doi: 10.3969/j.issn.1673-8225.2011.32.004

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Effect of DNA demethylation on proliferating cell nuclear antigen expression in bone marrow mesenchymal stem cells

Zhao Hong-xian1, Xu Fu-cui1, Wang Qiao-zhi1, Xu Qiang1, Chen Xia2   

  1. 1Department of Histology and Embryology, Luzhou Medical College, Luzhou  64600, Sichuan Province, China
    2Department of Digestive Disease, Affiliated Hospital of Luzhou Medical College, Luzhou  64600, Sichuan Province, China
  • Received:2011-02-18 Revised:2011-03-13 Online:2011-08-06 Published:2011-08-06
  • Contact: Chen Xia, Master, Department of Digestive Disease, Affiliated Hospital of Luzhou Medical College, Luzhou 64600, Sichuan Province, China flygirl0012000@yahoo.com.cn
  • About author:Zhao Hong-xian★, Master, Lecturer, Department of Histology and Embryology, Luzhou Medical College, Luzhou 64600, Sichuan Province, China zilong5276@sohu.com

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Abstract:

BACKGROUND: DNA demethylation is an important epigenetic modification.
OBJECTIVE: To explore the effect of DNA demethylation on proliferation and proliferating cell nuclear antigen (PCNA) protein expression in bone marrow mesenchymal stem cells.
METHODS: Bone marrow mesenchymal stem cells were isolated by using the method of adhesive culture of whole bone marrow. In the third passage bone marrow mesenchymal stem cells, CD44, CD45 and PCNA protein were dectcted by immunocytochemistry, and effect of DNA demethylation on proliferation was detected by MTT method.
RESULTS AND CONCLUSION: ①The third passage bone marrow mesenchymal stem cells expressed CD44 postively and CD45 negatively. ②Compared with control group, after 48-hour intervention of 5-azacytidine, 5-azacytidine with 6, 12,
24 μmol/L increased proliferation of cells without concentration-dependence significantly (P < 0.05). ③ Compared with control group, after 48-hour intervention of 5-azacytidine, 5-azacytidine with 6, 12, 24 μmol/L increased PCNA protein expression significantly in bone marrow mesenchymal stem cells without concentration-dependence (P < 0.05). In certain concerntration, 5-azacytidine can increase PCNA protein expression thirough DNA demethylation, which is the key to improve proliferation of bone marrow mesenchymal stem cells.

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