Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (32): 5909-5912.doi: 10.3969/j.issn.1673-8225.2011.32.005

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Differentiation of bone marrow mesenchymal stem cells into thyroid cells

Xin Qun, Liu Dong-yuan, Zhang Qin   

  1. Department of General Surgery, the 401 Hospital of Chinese PLA, Qingdao  266000, Shandong Province, China
  • Received:2011-03-29 Revised:2011-06-03 Online:2011-08-06 Published:2011-08-06
  • Contact: Zhang Qin, Associate chief physician, Department of General Surgery, the 401 Hospital of Chinese PLA, Qingdao 266000, Shandong Province, China Correspondence to: Liu Dong-yuan, Physician, Department of General Surgery, the 401 Hospital of Chinese PLA, Qingdao 266000, Shandong Province, China
  • About author:Xin Qun☆, Doctor, Attending physician, Department of General Surgery, the 401 Hospital of Chinese PLA, Qingdao 266000, Shandong Province, China xinqun@126.com, xulili201314@126.com

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Abstract:

BACKGROUND: Under certain conditions, rat bone marrow mesenchymal stem cells (BMSCs) can be induced to differentiate into thyroid cells.
OBJECTIVE: To establish the method to in vitro induce the differentiation of rat BMSCs into thyroid cells.
METHODS: BMSCs were separated using density gradient method, and induced with thyroid-stimulating hormone and insulin. A group without any induced materials was used as parallel control. Morphology changes were observed with inverted optical microscope and transmission electron microscopy, immune fluorescence detection method was applied for BMSCs differentiation.
RESULTS AND CONCLUSION: Thyroid cell-specific gene expression such as TSHr could be seen in cultured cells after 7 days; thyroid cell-differentiated cell markers TTF-1 expression was detected after 9 days; no change in the control group. BMSCs can be induced to differentiate into thyroid cells based on morphology and biological examination.

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