Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (50): 9387-.doi: 10.3969/j.issn.1673-8225.2010.50.019

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Effects of roubin-manipulation on knee joint chondrocyte apoptosis and proliferating cell nuclear antigen expression in rabbits

Ou Guo-jian1, Dai Qi-yi2, Qin Xue-liu2, Lin Guang-qi3, Liu Jing3, Teng Ju-zan2, Yao Ping2, Zhu Jiang-long2, Rong Xiang-bin2, Han Jie2, Xin Xiao-dong3, Yuan Jing-yang3   

  1. 1 Department of Orthopaedic Traumatology, Hezhou Hospital, Guangxi Traditional Chinese Medicine College, Hezhou  542500, Guangxi Zhuang Autonomous Region, China; 2 Department of Orthopedics, Ruikang Hospital, Guangxi Traditional Chinese Medicine College, Nanning  530011, Guangxi Zhuang Autonomous Region, China; 3 Guangxi Traditional Chinese Medicine College, Nanning  530001, Guangxi Zhuang Autonomous Region, China
  • Online:2010-12-10 Published:2010-12-10
  • Contact: Dai Qi-yi, Master, Professor, Department of Orthopedics, Ruikang Hospital, Guangxi Traditional Chinese Medicine College, Nanning 530011, Guangxi Zhuang Autonomous Region, China DIQY3146423@163.com Qin Xue-liu, Associate professor, Department of Orthopedics, Ruikang Hospital, Guangxi Traditional Chinese Medicine College, Nanning 530011, Guangxi Zhuang Autonomous Region, China qinxueliu 2008@163.com
  • About author:Ou Guo-jian★, Studying for master’s degree, Physician, Department of Orthopaedic Traumatology, Hezhou Hospital, Guangxi Traditional Chinese Medicine College, Hezhou 542500, Guangxi Zhuang Autonomous Region, China ogj321@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30860359*; the Natural Science Foundation of Science and Technology Commission of Guangxi, No. 0832178*

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Abstract:

BACKGROUND: Studies have demonstrated that manipulation and joint passive movement can promote self-repair of surrounding tissues. However, it is poorly understood whether manipulation has effect on knee joint chondrocyte apoptosis and proliferating cell nuclear antigen expression (PCNA).
OBJECTIVE: To observe the effect of roubin-manipulation on knee joint chondrocyte apoptosis and PCNA expression.
METHODS: New Zealand rabbits were randomly divided into 5 groups: manipulation group, sodium hyaluronate group and model contrast group were established high intraosseous pressure models of right leg by ligating the vena femoralis, gluteae inferiors or great saphenous vein; sham surgery group only cut out the skin and the normal group. One week later, the manipulation group was treated with roubin-manipulation for 5 weeks with 17 times. The sodium hyaluronate was injected into the intra-articular of sodium hyaluronate group once a week for 5 weeks. Cartilage sections of right knee-joint were analyzed by in situ end labeling and immunohistochemistry at the end of 12 weeks.
RESULTS AND CONCLUSION: Compared with the model group, the apoptosis cell index of the manipulation group was smaller (P < 0.01) but the PCNA expression was greater (P < 0.01). The results demonstrated that roubin-manipulation can decrease the apoptosis of chondrocyte and increase chondrocyte proliferation. 

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