Abstract:

BACKGROUND: Huoxue No. Ⅰ decoction comprises figwort root, ophiopogonis, angelica root and safflower which can prevent deep vein thrombosis of lower limb following prosthetic replacement.
OBJECTIVE: To explore the mechanism of action of Huoxue No. Ⅰ decoction by observing its effect on fibrinolytic function and vein endothelial structure in rats with deep venous thrombosis..
METHODS: Animal models of deep venous thrombosis the inferior caval veins were prepared by ligation and treated by Huoxue No. Ⅰ decoction with different doses, Mailuoshutong and physiological saline, respectively. Meantime, sham-surgery group was established. The thrombus wet weight, plasma tissue-type plasminogen activator (t-PA), plasminogen activator inhibitor (PAI) and the endotheliocyte of the inferior caval veins were detected.
RESULTS AND CONCLUSION: Compared with the model control group, Huoxue No. Ⅰ decoction with different doses groups could dramatically decreased wet weight of thrombus (P < 0.05 or P < 0.01), but increased serum t-PA contents (P < 0.05, P < 0.01); at 1, 3 and 6 days after high-dose of Huoxue No. Ⅰ decoction treatment, at 3 and 6 days after middle-dose of Huoxue No. Ⅰ decoction treatment or at 3 days after low-dose of Huoxue No. Ⅰ decoction treatment, the serum PAI levels were obviously decreased (P < 0.05). Compared with the model control group, the rough endoplasmic reticulum was obviously and mitochondria displayed condensed state at 1 and 3 days in the low- and high-dose of Huoxue No. Ⅰ decoction groups, and the collagen fiber began increasing at 6 day after operation. The findings suggest that, Huoxue No. Ⅰ decoction can inhibit deep venous thrombosis formation and protect vascular endothelial cells via increasing t-PA and inhibiting PAI activities.