Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (45): 8473-8475.doi: 10.3969/j.issn.1673-8225.2010.45.026

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A simple and effective method for single cells separation

Huang Ming-wei1, Lu Yun-fei2, Shen Gui-xin1, Li Tao1　   

  1. 1 Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China; 2 Surgical Department of Intestines-Stomach and Glandular Organ, First Affiliated Hospital, Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autnomous Region, China　
  • Online:2010-11-05 Published:2010-11-05
  • Contact: Lu Yun-fei, Master, Chief physician, Surgical Department of Intestines-Stomach and Glandular Organ, First Affiliated Hospital, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autnomous Region, China
  • About author:Huang Ming-wei☆, Doctor, Attending physician, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
  • Supported by:

    the Postgraduate Education Innovation Project of Guangxi Zhuang Autonomous Region, No. 2008105981002D33*

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Abstract:

BACKGROUND: Research of stem cell or cancer stem cell at single cell level would be popularity in the near further. Many researchers stop going ahead when they have no idea to obtain lots of single cells in their study. A simple and effective method for single cell separation would be a foundation of exactly and quantitative study at single cell level.
OBJECTIVE: To introduce a simple and effective method for single cell separation.
METHODS: The separation method was improved based on micromanipulate and single cells were separated by using special separation equipment which makes up of micropipettor and the smallest intravenous needle. Success rates of separation were evaluated by separated staining of single cells.
RESULTS AND CONCLUSION: Success rate of single cells separation was 96.5%. The method has many advantages: material was easily to find; the manipulate method was simple, visual, reliable and easy to popularization and application; high success rate. Those single cells which had been separated can be used for further study directly.

CLC Number: