Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (45): 8469-8472.doi: 10.3969/j.issn.1673-8225.2010.45.025

Previous Articles     Next Articles

Establishing a rat model of intramyocardial stem cell transplantation without artificial ventilation

Wei Feng, Zhang Jing, Wang Ting-zhong, Ni Ya-juan, Ma Ai-qun   

  1. Department of Cardiovascular Medicine, First Affiliated Hospital, Medical School, Xi’an Jiaotong University; Shaanxi Province Key Laboratory of Molecular Cardiology; Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi’an  710061, Shaanxi Province, China
  • Online:2010-11-05 Published:2010-11-05
  • Contact: Ma Ai-qun, Doctor, Professor, Department of Cardiovascular Medicine, First Affiliated Hospital, Medical School, Xi’an Jiaotong University; Shaanxi Province Key Laboratory of Molecular Cardiology; Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi’an 710061, Shaanxi Province, China maaiqun@medmail.com.cn
  • About author:Wei Feng☆, Doctor, Physician, Department of Cardiovascular Medicine, First Affiliated Hospital, Medical School, Xi’an Jiaotong University; Shaanxi Province Key Laboratory of Molecular Cardiology; Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi’an 710061, Shaanxi Province, China wf_cn2000@sina.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30800455*; the Research Fund for the Doctoral Program of Higher Education of China, No. 200806981027*; the Youth Science Foundation,No.81000063*

PDF

424

Abstract:

BACKGROUND: Models of intramyocardial transplantation of stem cells in small experimental animals are often established with artificial ventilation by endotracheal intubation, in addition to cutting sternum or ribs. The operation is seriously wounded and complicated, which may impact on survival of models.
OBJECTIVE: To establish a model of intramyocardial transplantation of stem cells in normal and infarcted rats without artificial ventilation.
METHODS: Sprague Dawley rats were assigned to two groups. Myocardial infarction group: rats, following successful establishment of myocardial infarction model, received bone marrow mesenchymal stem cells (BMSCs) transplantation. For each rat, BMSCs were injected into surrounding sites of the infracted region by five points. In normal group, BMSCs with the same quantity were injected into epicardial sites in the left ventricle anterior wall by the same method. Rats in each group were killed 4 days after transplantation, and hearts were obtained for frozen sections. The distribution and survival of BMSCs labeled with green fluorescent protein (GFP) were observed under fluorescence microscope. Immunofluorescence staining was used to examine the cardiac-specific proteins expression on BMSCs.
RESULTS AND CONCLUSION: The survival rate was 80% in transplantation model in myocardial infarction group, and 93% in normal group. 4 days after transplantation, a lot of BMSCs, which showed zonal distribution with arranging in parallel with myocardial fibers, could be observed in the transplantation area. Furthermore, Connexin43 and myosin heavy chain MYH were also detected on BMSCs. Results indicate that using this method, a model of intramyocardial transplantation of stem cells in rat can be established simply and effectively without using artificial ventilation.

CLC Number: