Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (45): 8464-8468.doi: 10.3969/j.issn.1673-8225.2010.45.024

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Isolation and culture of human submandibular gland stem/progenitor cells

Wang Chun-yu 1, 2, Huang Gui-lin1, Zhang Ni-ni1, Zhang Yue-rong1, Song Qing-gao1, Pan Guang-hua1, Fang Ning3   

  1. 1 Department of Oral and Maxillofacial Surgery, Stomatological Hospital, Zunyi Medical College, Zunyi  563003, Guizhou Province, China; 2 Department of Oral and Maxillofacial Surgery, Zunyi Municipal Stomatological Hospital, Zunyi  563000, Guizhou Province, China; 3 Guizhou Provincial Key Laboratory of Cell Engineering, Zunyi Medical College, Zunyi  563003, Guizhou Province, China
  • Online:2010-11-05 Published:2010-11-05
  • Contact: Huang Gui-lin, Doctor, Professor, Department of Oral and Maxillofacial Surgery, Stomatological Hospital, Zunyi Medical College, Zunyi 563003, Guizhou Province, China chaojiehuanghgl@163.com
  • About author:Wang Chun-yu★, Master, Physician, Department of Oral and Maxillofacial Surgery, Stomatological Hospital, Zunyi Medical College, Zunyi 563003, Guizhou Province, China; Department of Oral and Maxillofacial Surgery, Zunyi Municipal Stomatological Hospital, Zunyi 563000, Guizhou Province, China chun5873@126.com
  • Supported by:

     the Natural Science Research Program of Guizhou Province in 2009, No. 20090112*

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Abstract:

BACKGROUND: Recently, scholars have verified that salivary gland cells that have adult stem cell characteristics exist in salivary gland tissues. However, these stem cells mainly focus in animal salivary gland. There are a few studies concerning biological characteristics of human salivary gland stem cells.
OBJECTIVE: To isolate and culture human salivary gland stem/progenitor cells in vitro, and to investigate the biological characteristics of these cells.
METHODS: Human salivary gland stem/progenitor cells were harvested from submendubular glands of the legally abortion fetus for monoclonal culture. Epithelial cell marker cytokeratin 19 (CK19) and Laminin were analyzed by immunohistochemistry. Surface markers CD29, CD90.1 and CD117 expressions were detected by flow cytometric analysis. Growth curve of human salivary gland stem/progenitor cells was drawn, and proliferation ability in vitro was evaluated.
RESULTS AND CONCLUSION: The human salivary gland stem/progenitor cells expressed intracellular Laminin and CK19. Flow cytometric analysis results revealed that the percentage of cells positive for CD90.1, CD29 and CD117 were 99.69%, 98.83% and 67.27%. The cells had perfect ability of proliferation, clonality and passage in vitro. These indicate that these cells from fetus submandibular glands have the characteristics of tissue stem cells.

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