Abstract:

BACKGROUND: Hemopoietic stem cells have the potential of self replication and renewal. CD34⁺ cells have the marker of hemopoietic stem cells.
OBJECTIVE: To analyze the related factors that affect the peripheral blood CD34⁺ cell purification.
METHODS: From 90 cases of patients, the peripheral blood were collected, after human granulocyte colony stimulating factor (G-CSF) 5 μg/kg•d mobilizing for 1-3 days, using COBE machine mononuclear cells fluid 80-100 mL was collcected, the CD34⁺  stem cells were purified by Clini MACS.
RESULTS AND CONCLUSION: The mean value of CD34⁺ cells was (1.73±1.15)×10⁷ in 90 cases. Using flow cytometry, the positive rate of CD34⁺ cells was more than 80%. When the circulation blood liquid singly collected by COBE machine was 980- 1 100 mL, it was useful for CD34+ cells collection (P = 0.005). After mobilizing, when the white blood cell concentration was (16-21)×109/L, it was useful for CD34⁺ cells collection (P < 0.05);  when the general ratio of middle cells to lymphocytes was more than 11%, it was useful for CD34⁺ cell collection (P < 0.05).  When the concentration of blood platelet in mononuclear cells was less than 2 100×10⁹/L, it was useful for CD34+ cell collection (P < 0.05); when the patient’s age was younger than 16 years, the number of collected CD34⁺ cells was high (P = 0.003). Moreover, the temperature of CD34⁺ cells, the magnetism marker, the eccentricity of treating cells, all could affect the CD34⁺ cells collection. Results indicate that the CD34⁺ cells purified by Clini MACS machine could fit the clinical need and keep good experiment stability and repeatability. To improve the CD34⁺ cells collection, the related factors which affect the peripheral blood CD34⁺ purification should be carefully considered.