Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (45): 8361-8364.doi: 10.3969/j.issn.1673-8225.2010.45.001

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Wnt signaling molecules induce the differentiation of bone marrow mesenchymal stem cells into neuron-like cells

Tang Yu1, Ma Yun-sheng2, Qin Shu-jian2　   

  1. 1 Department of Neurology, Beijing Fuxing Hospital, Beijing  100000, China; 2 Department of Histology and Embryology, Liaoning Medical University, Jinzhou  121000, Liaoning Province, China　
  • Online:2010-11-05 Published:2010-11-05
  • Contact: Ma Yun-sheng, Lecturer, Department of Histology and Embryology, Liaoning Medical University, Jinzhou 121000, Liaoning Province, China yunshengma@126.com
  • About author:Tang Yu★, Master, Attending physician, Department of Neurology, Beijing Fuxing Hospital, Beijing 100000, China
  • Supported by:

     the Grant of Education Bureau of Liaoning Province, No. 2008403*

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Abstract:

BACKGROUND: Mesenchymal stem cells (MSCs) could differentiate into nerve cells, but the molecular mechanism is still unknown.
OBJECTIVE: To observe effects of Wnt3a and Wnt5a on differentiation of bone marrow mesenchymal stem cells (BMSCs) into nerve cells.
METHODS: The rat BMSCs were separated and cultured in vitro. Following passage, expression of CD14, CD44, CD9, CD34 and CD45 was detected by morphology and flow cytometry. Wnt3a, Wnt5a contained with basic fibroblast growth factor (bFGF) were used to induce the differentiation of BMSCs into neuron-like cells. Wnt3a and Wnt5a effects were identified by immunohistochemistry and RT-PCR.
RESULTS AND CONCLUSION: BMSCs were fibroblast-like. CD9 and CD44 were highly expressed, but CD34 and CD45 low expressed. Nestin and neuron specific enolase (NSE) were positive and glial fibrillary acidic protein (GFAP) was not positive when they was cultured with Wnt3a. In Wnt5a group, Nestin was weak positive, but NSE and GFAP were negative. The RT-PCR results revealed Nestin expressed before and after induction; NSE was significant at 5 days following induction, especially at 10 days; weak GFAP expression was detectable at 10 days in Wnt3a group. These indicated that Wnt3a can promote the differentiation of BMSCs into neuron-like cells.

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