Abstract:

BACKGROUND: Schwann cells have not yet a large number of division and proliferationin following early peripheral nerve injury. As anatomic discontinuity, the nutritional factors through axoplasmic retrograde transport reduce sharply. The neurons are likely to die due to lack of neurotrophic factors support, so peripheral nerves cannot regenerate or show weak regeneration.
OBJECTIVE: To evaluate the effects of the implantation of bone marrow mesenchymal stem cells (BMSCs) induced by the platelet-rich plasma (PRP) combined with chemical extraction of acellular nerve on repairing sciatic nerve defect.
METHODS: New Zealand white rabbits were selected to establish sciatic nerve defect models, and randomly divided into four groups, namely pure chemical extracted acellular nerve group, transplantation of allogenic acellular nerve; BMSC group, transplantation of allogenic BMSCs combined with chemical extraction of allogenic acellular nerve; induced BMSC group, transplantation of the PRP-induced allogenic BMSCs combined with chemical extraction of allogenic acellular nerve; and autologous nerve group, transplantation of autologous nerve. Detection indicators include morphological observation, the target muscle recovery rate of muscle wet weight, motor nerve conduction velocity (MNCV) and the axon diameter and myelin sheath thickness.
RESULTS AND CONCLUSION: Target muscle to restore the rate of muscle wet weight, MNCV, axon diameter, myelin sheath thickness and the morphology in PRP induced by BMSCs combined with chemical extraction of acellular nerve group was significantly superior to a pure chemical extracted acellular nerve group and BMSCs combined with chemical extraction of acellular nerve group, but there were similar with autologous nerve repair group. BMSCs have the function of Schwann cells in vivo after induction. BMSCs can be used as tissue-engineered peripheral nerve of the seed cells for the repair of peripheral nerve defects