Effect of collagen scaffold on proliferation and osteogenic
differentiation of human periodontal ligament stem cells treated by Eucommia
ulmoides Oliver leaf extract

doi:10.3969/j.issn.2095-4344.2220

Abstract

Abstract:

BACKGROUND: Collagen scaffold is a good tissue engineering material. However, there are no reports on the effects of collagen scaffold on the biocompatibility, proliferation, and osteogenic activity of human periodontal ligament stem cells (hPDLSCs) treated by Eucommia ulmoides Oliver leaf extract.

OBJECTIVE: hPDLSCs treated by Eucommia ulmoides Oliver leaf extract were co-cultured with collagen scaffolds in vitro to investigate the morphological characteristics, adhesion, proliferation and osteogenic differentiation of hPDLSCs on collagen scaffolds.

METHODS: In the experimental group, hPDLSCs treated by Eucommia ulmoides Oliver leaf extract were co-cultured with collagen scaffolds in vitro. In the positive control group, hPDLSCs were co-cultured with collagen scaffolds in vitro. In the blank control group, only hPDLSCs were cultured.

RESULTS AND CONCLUSION: Scanning electron microscopy results revealed that in the experimental group, a large number of hPDLSCs adhered to the scaffold, and the synapses of the cells were closely connected with the scaffold. The cells grew well and contacted closely with each other. After co-culture for 4, 8 and 12 hours, cell adherence rate in both experimental and positive control groups increased and it in the experimental group was significantly higher than that in the positive control group (P < 0.05). MTT results showed that after 3, 5 and 7 days of co-culture, absorbance value in the experimental group was significantly higher than that in the positive control group (P < 0.05). After 7 and 14 days of osteogenic induction, alkaline phosphatase activity in the experimental group was significantly higher than that in the positive control group (P < 0.05). RT-PCR results showed that after 21 days of osteogenic stimulation, the expression of osteogenesis-related genes Runx2, OPN and OCN in the experimental group was significantly higher than that in the blank control group (P < 0.01), and the expression of the above genes in the experimental group was higher than that in the positive control group (P < 0.05). These data indicate that collagen scaffolds have good biocompatibility with hPDLSCs treated by Eucommia ulmoides Oliver leaf extract, can maintain cell morphology, and promote cell proliferation and osteogenic differentiation after co-culture.

Key words: stem cells, human periodontal ligament stem cells, co-culture, cell identification, biocompatibility, proliferation, osteogenic induction

CLC Number:

Liu Yan, Chen Qingyu, Gao Xiang, Gao Junwu. Effect of collagen scaffold on proliferation and osteogenic differentiation of human periodontal ligament stem cells treated by Eucommia ulmoides Oliver leaf extract[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(16): 2537-2543.

Figures/Tables 8

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