Anti-osteoarthritis activity of urtica extracts at different concentrations

doi:10.3969/j.issn.2095-4344.2017.36.001

Abstract

Abstract:

BACKGROUND: Urtica, as a traditional Chinese medicine, is famous for anti-inflammatory, detumescence and anti-rheumatic effects, but its effect on osteoarthritis remains unknown.
OBJECTIVE: To investigate the therapeutic effect of different concentrations of urtica on osteoarthritis and the underlying mechanism.
METHODS: (1) Eighteen rats were selected, and after their hair was shaved, the rats were divided into three groups (n=6 per group). The rat skin was painted with paraffin, urtica colloid and 0.8% formaldehyde, respectively, and the skin rash and swelling were observed. (2) Thirty rats were selected, and the rat model of osteoarthritis was established by injecting papain, followed by equally allotted into 100, 200, and 400 mg/kg urtica and positive control groups. The diameter of the rat left knee was measured at 1, 2 and 3 weeks after administration to determine the severity of knee swelling. The rat model of acute pain was established with 0.6% acetic acid solution, and then the model rats were subjected to different concentrations of urtica or normal saline (control group). (3) The frequency of rat tumbling and twisting was observed to determine the analgesic effect of urtica. The levels of interleukin-1β, tumor necrosis factor-α and matrix metalloproteinase 3 in the synovial fluid of the left knee joint were detected by ELISA at 1, 2 and 3 weeks after administration.
RESULTS AND CONCLUSION: After urtica administration, no edema or rash appeared on the rat skin surface. Compared with the control group, 200 and 400 mg/kg urtica could reduce joint swelling and pain, showing no significant difference from indomethacin. Compared with the control group, 200 and 400 mg/kg urtica reduced the levels of interleukin-1β, tumor necrosis factor-α and matrix metalloproteinase 3 in the synovial fluid. These results manifest that urtica exerts the effects of anti-inflammation, analgesia, detumescence and anti-osteoarthritis.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Urticaria, Osteoarthritis, Interleukin-1beta, Tumor Necrosis Factor-alpha, Matrix Metalloproteinase 3, Tissue Engineering

CLC Number:

R318

Zheng Xiao-fen. Anti-osteoarthritis activity of urtica extracts at different concentrations[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(36): 5741-5746.

Figures/Tables 2

References

[1] 王梦月,刘瑛,卫莹芳,等. 川产荨麻药理作用的初步研究[J]. 中国民族民间医药杂志, 2001,51:229-231

[2] 王梦月,卫莹芳,史炎,等. 三种活麻水煎液药理作用探索[J]. 时珍国医国药, 2001, 12(8):673-674

[3] 王梦月,卫莹芳,李晓波. 荨麻抗风湿活性部位的化学成分研究[J]. 中草药,2006,37(9):1300-1303

[4] 王梦月,卫莹芳,史焱. 荨麻多糖药理作用初步研究[J]. 中药材, 2001,24(9):666-667

[5] 敖特根白音,李运起,韩艳华,等. 国内外麻叶荨麻的研究进展[J]. 中国野生植物资源, 2015,34(1):32-36

[6] 张嫚丽.麻叶荨麻化学成分及抗炎药理活性初步研究[D].河北医科大学, 2004.

[7] 李喆: 麻叶荨麻叶化学成分、多糖含量测定及抗炎活性的研究[J]. 河北医科大学, 2008.

[8] Randall C, Meethan K, Randall H, et al. Nettle sting of Urtica dioica for joint pain — an exploratory study of this complementary therapy. Complement Ther Med. 1999;7(3): 126-131.

[9] 李妍.荨麻多糖提取分离、结构分析及功能的研究[D].中国人民解放军军事医学科学院, 2005.

[10] 李婉. 浅谈非甾体抗炎药的不良反应及其预防[J]. 中国保健营养,2017,27(10):56-57

[11] 李冬,张光武,刘家帮,等. 非甾体抗炎药辅助关节镜治疗膝骨关节炎的临床疗效分析[J]. 临床药物治疗杂志, 2015, 13(4): 54-57

[12] LUU THI THANH THUY , 王梦月. 荨麻属药用植物的研究进展[J].中国民族民间医药, 2015,12:16-24.

[13] 王燕雲,刁治民,曹霞,等. 药用植物荨麻经济价值及应用潜力[J]. 青海草业,2015,24(2):25-34

[14] 李晓红,赵永娜,李顺英,等. 滇产粗根荨麻不同提取部位的镇痛抗炎作用研究[J]. 天然产物研究与开发, 2008,20(2):343-345.

[15] 刘敏, 陈阳斌. 依托考昔治疗骨关节炎研究进展[J]. 现代仪器与医疗, 2016, 22(4):1-3

[16] 肖壮,唐涛,孙先润,等. 骨关节炎治疗药物的研究进展[J].中国药房,2016,27(35):5037-5040

[17] 石劲敏,李瑞. 手性非甾体抗炎新药依托度酸[J]. 中国临床药学杂志,2000,9(5):321-324.

[18] van Ryn J, Pairet M.Selective cyclooxygenase-2 inhibitors: pharmacology, clinical effects and therapeutic potential. Expert Opin Investig Drugs.1997;6(5):609-614.

[19] 薛静,伍骥,郑超. 基于循证医学指导下的骨关节炎治疗建议[J]. 中华关节外科杂志,电子版:2013,7(3):2083-2086.

[20] 任硕. 膝骨关节炎治疗方案的优化选择[J]. 山东中医药大学, 2010.

[21] Hochberg MC, Altman RD, April KT,et al.American college of Rheumatology 2012 recomnlendations for the use of nonpharmacologic and phamacologictherapies in osteoarthritis of the hand,hip,and knee. Arthritis Care Res (Hoboken). 2012;64(4):465-474.

[22] Wu MX, Li XH, Lin MN, et al.clinical study on the treatment of Knee osteoarthritis of Shen-Sui insufficiency syndrometype by electmacupuncture. Chin J Integr Med.2010;16(24): 291-297．

[23] 崔殿波,李薇,王莹,等. 狭叶荨麻提取物抗炎镇痛药理作用研究[J]. 中医药学报, 2014,42(4):61-63.

[24] 王亚丽,秦民坚,戴岳,等.狭叶荨麻根、茎、叶的抗炎镇痛作用[J].现代中药研究与实践, 2005,19(3):42-43,46.