Co-culture with vascular endothelial progenitor cells: effects on proliferation and apoptosis of neural stem cells and vascular remodeling in rats with ischemia reperfusion injury

doi:10.3969/j.issn.2095-4344.2017.05.011

Abstract

Abstract:

BACKGROUND: Neural stem cell (NSC) transplantation is a common method for various ischemic
encephalopathies, but inability to survive in the transplantation region limits its further use in clinical practice.
OBJECTIVE: To explore the effect of vascular endothelial progenitor cells (VEPCs) on the proliferation and apoptosis of co-cultured NSCs as well as vascular remodeling in rats with ischemia reperfusion injury.
METHODS: 125 Sprague-Dawley rats were randomly divided into five groups, 25 rats in each group, including sham operation, ischemia, NSCs, co-culture, and VEPCs groups. Rat models of ischemia reperfusion injury were made in all groups except for the sham operation group, followed by corresponding interventions. The proliferation and apoptosis of neural stem cells were detected, and vascular remolding in the ischemic region was observed in each group.
RESULTS AND CONCLUSION: At different time points after transplantation, BrdU positive cells were not observed in VEPCs, ischemia and sham operation groups; the number of BrdU positive cells in the co-culture group was significantly higher than that in the NSCs group (P < 0.05); BrdU+/Caspase-3+ cell were observed in both co-culture and NSCs groups, and the apoptosis rate of the co-culture group was significantly lower than that in the NSCs group (P < 0.05); there were new blood vessels in all the groups except for the sham operation group, and the number of new bone vessels was highest in the co-culture group. To conclude, our experimental results show that VEPCs promotes the proliferation of co-cultured NSCs, inhibits cell apoptosis and and promote angiogenesis in the ischemic penumbra of rats with ischemia reperfusion injury.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Reperfusion Injury, Neural Stem Cells, Cell Proliferation, Apoptosis, Neovascularization, Physiologic, Tissue Engineering

CLC Number:

R394.2

Yang Chun-sheng, He Dan, Tan Jun. Co-culture with vascular endothelial progenitor cells: effects on proliferation and apoptosis of neural stem cells and vascular remodeling in rats with ischemia reperfusion injury[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(5): 718-723.

Figures/Tables 3

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