Chinese Journal of Tissue Engineering Research ›› 2017, Vol. 21 ›› Issue (4): 580-585.doi: 10.3969/j.issn.2095-4344.2017.04.015

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Effects of vascular endothelial growth factor combined with basic fibroblast growth factor on periodontal ligament fibroblast proliferation and alkaline phosphatase activity in rats

Cao Yu1, Wang Li-li2   

  1. 1Department of Stomatology, the Sixth People’s Hospital of Shenyang, Shenyang 110000, Liaoning Province, China; 2 Department of Prosthodontics, the Affiliated Stomatological Hospital of Jinzhou Medical University, Jinzhou 121004, Liaoning Province, China
  • Received:2016-12-12 Online:2017-02-08 Published:2017-03-13
  • Contact: Corresponding author: Wang Li-li, M.D., Associate chief physician, Department of Prosthodontics, the Affiliated Stomatological Hospital of Jinzhou Medical University, Jinzhou 121004, Liaoning Province, China
  • About author:Cao Yu, Associate chief physician, Department of Stomatology, the Sixth People’s Hospital of Shenyang, Shenyang 110000, Liaoning Province, China
  • Supported by:

    the National Science Foundation for the Talents of Liaoning Science and Technology Department, No. 2014022003; the Innovation Training Program of College Students of Liaoning Province, No. 201510160000045

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Abstract:

Abstract
BACKGROUND: Basic fibroblast growth factor (bFGF) can enhance fibroblast proliferation and collagen deposition, and vascular endothelial growth factor (VEGF) can improve blood perfusion and metabolic level of pathological tissues. Additionally, both of them can boost the alkaline phosphatase activity under given conditions.
OBJECTIVE: To evaluate the effect of bFGF combined with VEGF on the periodontal ligament fibroblast proliferation and alkaline phosphatase activity in rats.
METHODS: Rat periodontal ligament fibroblasts were cultured in vitro, its embryonic origin was identified and passage 4 cells were used for the following experiments. Effects of bFGF and VEGF with different concentrations on the rat periodontal ligament fibroblast proliferation were detected to determine the minimum and maximum effective concentrations. Cells were divided into five groups: group A (control group) with DMEM containing 2% fetal bovine serum; group B as maximum effective concentration of VEGF group; group C as maximum effective concentration of bFGF; group D as minimum effective concentration of bFGF combined with minimum effective concentration of VEGF group; group E as maximum effective concentration of bFGF combined with maximum effective concentration of VEGF group. At 3, 7 and 14 days, the alkaline phosphatase activity in each group was detected.
RESULTS AND CONCLUSION: Rat periodontal ligament fibroblasts derived from the mesoderm grew well. Rat periodontal ligament fibroblast proliferation was increased with the VEGF and bFGF concentration increasing (P < 0.01). The maximum and minimum effective concentrations of VEGF were 100 and 10 μg/L, and the maximum and minimum effective concentrations of bFGF were 10 and 0.1 μg/L. The absorbance values in the groups D and E were higher than those in the group A. The absorbance values of the group D were significantly lower than those of the group E at 3 and 7 days (P < 0.05), but did not significantly differ at 14 days (P > 0.05). To conclude, the combination use of the maximum effective concentration of VEGF and bFGF can play a significant synergistic effect on the alkaline phosphatase activity at a given time, but the minimum and maximum effective concentrations show no significant differences if not in the given time, which may be related to the time-effectiveness of these two factors and the receptors of periodontal ligament cells.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Fibroblast Growth Factor 2, Vascular Endothelial Growth Factors, Alkaline Phosphatase, Tissue Engineering

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