Three-dimensional printed silk fibroin/collagen scaffold and its performance

doi:10.3969/j.issn.2095-4344.2017.02.021

Abstract

Abstract:

BACKGROUND: Searching for a porous three-dimensional (3D) scaffold holding good porosity, mechanical property and biocompatibility has become a hot spot, in which, 3D printing technology also plays apart.
OBJECTIVE: To prepare silk fibroin/collagen scaffolds using 3D printing technology and detect its performance.
METHODS: Silk fibroin/collagen scaffolds were constructed using 3D printing technology, and the silk fibroin/collagen mass ratio was 4:2 (group A) and 4:4 (group B), respectively. The porosity, water absorption expansion rate, mechanical properties and pore size of the composite scaffolds were detected. The passage 3 rat bone marrow mesenchymal stem cells were seeded onto the two scaffolds. The cell proliferation was detected using MTT assay at 13 days of culture, and the cell morphology was observed by hematoxylin-eosin staining and scanning electron microscope at 14 days of culture.
RESULTS AND CONCLUSION: The porosity, pore size, and water absorption expansion rate of group A were significantly larger than those of group B (P < 0.05), while the elasticity modulus showed no significant difference between groups. Bone marrow mesenchymal stem cells on the two scaffolds increased gradually with time, especially in the group A (P < 0.05). Abundant cells distributed evenly in the group A, while few cells distributed unevenly in the group B. These results suggest that the 3D printed scaffolds composed by silk fibroin/collagen mass ratio of 4:2 holds good physicochemical performance and cytocompatibility.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Silk, Collagen, Tissue Engineering

CLC Number:

R318

Sun Kai, Li Rui-xin, Fan Meng, Li Yi-jin, Dong Bao-kang, Li Hui.

Three-dimensional printed silk fibroin/collagen scaffold and its performance

[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(2): 280-285.

Figures/Tables 6

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