Erk activation and proliferation in osteoarthritc chondrocytes after continuous passive motion

doi:10.3969/j.issn.2095-4344.2016.42.005

Abstract

Abstract:

BACKGROUND: Whether continuous passive motion improves osteoarthritis by enhancing the proliferation ability of chondrocytes is rarely reported.
OBJECTIVE: To analyze the therapeutic outcomes of continuous passive motion in rabbits with osteoarthritis and the underlying mechanism.
METHODS: Thirty-six New Zealand white rabbits were randomly allotted into three groups (n=12 per group). Rabbits in control group only underwent capsulotomy with no harm to the cartilage; osteoarthritis models were established in the rabbits of model and treatment groups using Hulth method. At 1 day after modeling, the treatment group rabbits were treated with continuous passive motion, 8 hours daily for consecutive 8 weeks. Interleukin-1 and tumor necrosis factor α levels in the synovial fluid were detected by ELISA; collagen type II expression and the proliferation ability of chondrocytes were detected by MTT assay; Erk signaling pathway activation was determined using western blot assay.
RESULTS AND CONCLUSION: In the model group, interleukin-1 and tumor necrosis factor α levels in the synovial fluid were significantly increased, and the expression level of collagen type II mRNA was remarkablely down-regulated. Continuous passive motion significantly downregulated interleukin-1 and tumor necrosis factor α levels and up-regulated the collagen type II mRNA level (P < 0.01). The model group showed significantly decreased proliferation ability of chondrocytes and down-regulated Erk signaling pathway activation, while after continuous passive motion, all above indicators were significantly improved (P < 0.01). These results indicate that the continuous passive motion can alleviate osteoarthritis probably by influencing interleukin-1 and tumor necrosis factor α levels, proliferation ability of chondrocytes, and collagen type II expression, as well as regulating Erk signaling pathway activation.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Motion Therapy, Continuous Passive, Osteoarthritis, Chondrocytes, Signal Tansduction, Tissue Engineering

CLC Number:

R318

Muyesaier•Maimaitiniyazi, Hu Yi, Ren Yun-ping, Zhang Yong, Xiong Dao-hai. Erk activation and proliferation in osteoarthritc chondrocytes after continuous passive motion[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2016.42.005.

Figures/Tables 3

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