Biological behavior of rat bone marrow mesenchymal stem cells in oligo(poly(ethylene glycol)fumarate) hydrogel

doi:10.3969/j.issn.2095-4344.2016.16.016

Abstract

Abstract:

BACKGROUND: Oligo(poly(ethylene glycol)fumarate) (OPF) hydrogel is a kind of biomaterial with good biocompatibility, injectability, and biodegradability. It is known that there are differences in the characteristics of hydrogels with different molecular weights. Under osteogenic induction, bone marrow mesenchymal stem cells encapsulated in the suitable molecular weight hydrogel have better proliferation and differentiation. Therefore, the use of OPF hydrogel provides new options for bone tissue-engineered scaffold.

OBJECTIVE: To investigate the effect of OPF hydrogel with different molecular weights on the proliferation and differentiation of encapsulated rat bone marrow mesenchymal stem cells in vitro.

METHODS: Four kinds of OPF hydrogels with molecular weights of 1 000, 3 000, 10 000 and 35 000 were developed by OPF crosslinking with a redox radical initiation system. Hydrogel swelling and degradation properties were detected. Afterwards, bone marrow mesenchymal stem cells encapsulated in different hydrogels were cultured in osteogenic medium for 1-3 weeks. The effects of hydrogels on morphplogy of bone marrow mesenchymal stem cells and osteogenic differentiation were detected via histological staining (hematoxylin-eosin staining and alizarin red staining) and immunofluorescence staining.

RESULTS AND CONCLUSION: With the increase of molecular weight of hydrogels, the gelation time was shortened, the swelling ratio significantly increased, and the hydrogel degradation rate was proportional to the molecular weight. In addition, the number of mineralized nodules formed in 3 000 and 10 000 molecular weight hydrogels was more than that in the other hydrogels, indicating that hydrogels with appropriate swelling and degradation properties are beneficial to cell proliferation and differentiation. In conclusion, OPF hydrogels have good biocompatibility and hydrogels with 3 000 and 10 000 molecular weight play active regulatory roles in osteogenic differentiation of mesenchymal stem cells.
中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Gels, Mesenchymal Stem Cells, Tissue Engineering

Wei Li-jun1, Cao Jun-kai1, Li Jun-jie2, Feng Lan-lan2 . Biological behavior of rat bone marrow mesenchymal stem cells in oligo(poly(ethylene glycol)fumarate) hydrogel[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(16): 2397-2404.

Figures/Tables 8

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