Combining bone marrow mesenchymal stem cells with new nerve conduits for repair of peripheral nerve defects

doi:10.3969/j.issn.2095-4344.2015.45.019

Abstract

Abstract:

BACKGROUND: With the continuous development and progress of medical model, the treatment and rehabilitation recovery from peripheral nerve defects needs higher requirements; therefore, stem cell culture-based nerve tissue engineering technology provides a new strategy for nerve defect repair.
OBJECTIVE: To explore the feasibility of combining bone marrow mesenchymal stem cells and new nerve conduits to repair peripheral nerve defects.
METHODS: Fifty New Zealand white rabbits, clean grade, were randomly divided into experimental group and control group, with 25 rabbits in each group. Animal models of peripheral nerve defects were made about 15 mm distant to the middle segment of the radius of the rabbit foreleg. At 1 week after modeling, bone marrow mesenchymal stem cells/new nerve conduit composite material was implanted into the defect in the experimental group; and in the control group, only bone marrow mesenchymal stem cells were transplantation. At 4 weeks after transplantation, a 5-mm nerve fiber was taken from the defect site, stained with hematoxylin-eosin and observed under scanning electron microscope. Density and diameter of regenerated nerve fibers were compared between the two groups.
RESULTS AND CONCLUSION: Compared with the control group, the density of nerve fibers was significantly higher in the experimental group, but the diameter of nerve fibers was significantly lower (P < 0.05). There were more cells with good growth, large size and many processes on the surface of regenerated nerve tissues in the experimental group than the control group. Moreover, in the experimental group, interconnected cells were woven into a mesh and the axons were longer and thicker, both of which were the performance of typical neuron-like cells. Taken together, the bone marrow mesenchymal stem cells/new nerve conduit composite material can be used for peripheral nerve repair and present exact achievements.
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cells, Peripheral Nerves, Wounds and Injuries, Tissue Engineering

Zhu De-zhi, Wang Bo-min. Combining bone marrow mesenchymal stem cells with new nerve conduits for repair of peripheral nerve defects[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(45): 7320-7324.

Figures/Tables 4

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