Bone marrow mesenchymal stem cells combined with chitosan for articular cartilage injuries

doi:10.3969/j.issn.2095-4344.2015.45.007

Abstract

Abstract:

BACKGROUND: Trauma easily leads to the emergence of articular cartilage defects, which is a difficult problem in the orthopedics field. Tissue engineering technology provides a new method for cartilage repair.

OBJECTIVE: To explore the feasibility of combining chitosan and bone marrow mesenchymal stem cells to repair injured articular cartilage in rabbits.

METHODS: Cultured rabbit bone marrow mesenchymal stem cells were seeded onto chitosan scaffold, and then the composite material was implanted into the defect as experimental group. Rabbits with no treatment served as control group. Gross observation and toluidine blue staining were carried out at 6 and 12 weeks after operation.

RESULTS AND CONCLUSION: At 6 weeks after operation, the control group had only fibrous tissue hyperplasia, and in the experimental group, cartilage-like tissues were generated at the defect site. At 12 weeks after operation, a small amount of hyaline cartilage-like tissues were observed in the control group, and the defects in the experimental group were covered with smooth and hyaline cartilage tissues. After 12 weeks, the toluidine blue staining was light in the control group with a small amount of cartilage tissues; in the experimental group, the toluidine blue staining was remarkable, and the defects were completely covered with hyaline cartilage tissues, and cartilage cells were increased in number. The findings indicate that chitosan-bone marrow mesenchymal stem cells composite material is better to induce cartilage tissue formation and promote cartilage defect repair.
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cells, Chitosan, Cartilage, Articular, Tissue Engineering

Chen Lu, Xia Xian-xue, Jiang Ke. Bone marrow mesenchymal stem cells combined with chitosan for articular cartilage injuries[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(45): 7254-7258.

Figures/Tables 6

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