Bone marrow mesenchymal stem cell conditioned medium with hypoxic activation enhances its effects on radiation-induced intestinal epithelial cell injury in vitro

doi:10.3969/j.issn.2095-4344.2015.45.003

Abstract

Abstract:

BACKGROUND: Conditioned medium from mesenchymal stem cells (MSC-CM) that contains abundant MSCs paracrine substances may represent a promising alternative to MSCs transplantation. However, normal MSC-CM with insufficient paracrine ability is not effective for tissue damage repair.
OBJECTIVE: To investigate the effects of MSC-CM with (MSC-CMHyp) and without hypoxic activation (MSC-CMNor) on the proliferation and apoptosis of radiation-induced injured intestinal epithelial cells (IEC-6) and to further discuss the paracrine mechanisms.
METHODS: IEC-6 cells were exposed to 10 Gy irradiation and cultured in MSC-CMHyp, MSC-CMNor, and DMEM-F12 medium, respectively.
RESULTS AND CONCLUSION: Findings from trypan blue staining, flow cytometry and western blot assay showed that, compared with the DMEM-F12 medium group, treatment with MSC-CMHyp significantly enhanced IEC-6 viability proliferation after radiation-induced injury, as well as significantly decreased cell apoptosis and expression of Caspases-3/8 (P < 0.05). However, there was no significant difference between the MSC-CMNor group and DMEM-F12 medium group (P > 0.05). On the other hand, the increased levels of vascular endothelial growth factor, basic fibroblast growth factor, insulin-like growth factor-1, and interleukin-10 were detected in the MSC-CMHyp group compared to the MSC-CMNor group (P < 0.05). These results suggest that the MSC-CMHyp improves the viability and proliferative capacity of IEC-6 cells after radiation-induced injury via up-regulating secretion of cytokines and down-regulating apoptotic signaling.
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Mesenchymal Stem Cells, Culture Media, Conditioned, Intestine, Small, Tissue Engineering

Zheng Yuei, Chen Hao, Sha Wei-hong, Wang Qi-yi, Liu Wan-wei. Bone marrow mesenchymal stem cell conditioned medium with hypoxic activation enhances its effects on radiation-induced intestinal epithelial cell injury in vitro[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(45): 7230-7236.

Figures/Tables 4

References

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