Effect of silk fibroin/hydroxyapatite scaffold on the viability and osteogenic properties of adipose-derived stem cells under osteogenic induction

doi:10.3969/j.issn.2095-4344.2015.45.002

Abstract

Abstract:

BACKGROUND: Adipose-derived stem cells under osteogenic induction can be combined with biodegradable silk fibroin/hydroxyapatite scaffold, which is expected to develop a new biocompatible and osteogenic bone fusion material.

OBJECTIVE: To study the effect of silk fibroin/hydroxyapatite composite on the viability and osteogenic properties of adipose-derived stem cells after osteogenic induction.

METHODS: Adipose-derived stem cells were obtained from rat’s fat tissue, then adherently cultured, proliferated and passaged in vitro. Passage 3 cells were cultured in conditioned medium for osteogenic induction, and then seeded onto silk fibroin/hydroxyapatite scaffold as experimental group. Adipose-derived stem cells cultured on the cover glasses at the same condition acted as control group. The cellular morphology, proliferation and differentiation were assessed respectively by means of phase contrast microscope, MTT assay and alkaline phosphatase activity measurement.

RESULTS AND CONCLUSION: After osteogenic induction, adipose-derived stem cells could adhere to the scaffold material and proliferate on the surface of silk fibroin/hydroxyapatite scaffold normally. No significant difference was found in cell proliferation and alkaline phosphatase activity between the experimental and control groups (P > 0.05), suggesting the cellular activity and function were not affected by the material. These findings indicate that silk fibroin/hydroxyapatite composite material has good cytocompatibility.
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Silk, Hydroxyapatites, Stem Cells, Adipose Tissue, Biocompatible Materials, Tissue Engineering

Liu Hao, Chu Ya-wei, Ding Tao, Cheng Li, Zhu Hao-ming. Effect of silk fibroin/hydroxyapatite scaffold on the viability and osteogenic properties of adipose-derived stem cells under osteogenic induction[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(45): 7224-7229.

Figures/Tables 11

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