Construction and evaluation of acute hind limb ischemia model in rats

doi:10.3969/j.issn.2095-4344.2015.40.018

Abstract

Abstract:

BACKGROUND: The limb ischemia model in rats has been widely applied in China to study the pathological process and treatment method of limb ischemia, but there are some controversies on the model construction and evaluation, so a reliable, convenient and economical disease model is needed for the related research.

OBJECTIVE: To compare limb ischemia degree, duration and changing rule of acute hind limb ischemia models prepared by different ways in Sprague-Dawley rats, and find a method to prepare models that have moderate and stable limb ischemia and maintain a longer time.

METHODS: 72 Sprague-Dawley rats were randomly divided into four groups, with 18 rats in each group. Group A: sham operation group, abdominal aorta below renal artery and iliolumbar artery, right superficial femoral artery, popliteal artery, saphenous artery were separated. Group B: the right superficial femoral artery, popliteal artery, saphenous artery were cut off, and the right femoral artery was resected to establish acute hind limb ischemic model. Group C: abdominal aorta and bilateral abdominal perineal artery were ligated to establish acute hind limb
ischemic model. Group D: abdominal aorta, iliolumbar artery and lumbar artery were ligated to establish acute hind limb ischemic model.

RESULTS AND CONCLUSION: Right hind limb muscle strengths of rats in groups B, C, D were weaker than that in group A at weeks 2, 4, 6 after operation; muscle strengths in group D was weaker than that in groups B, C at week 4 after operation; muscle strengths in groups B, D was still weaker than that in group C at week 6 after operation. The partial venous oxygen pressure of right hind limbs in groups B, C, D was lower than that in group A at weeks 2, 4, 6 after operation; the partial venous oxygen pressure in group D was lower than that in groups B and C at weeks 2, 4 after operation; and group D was still lower than group C at week 6 after operation. At weeks 2, 4 after operation, some muscle cells in the right hind limb muscle tissue were disrupted, fibrous connective tissue hyperplasia and capillary hyperplasia were detected, inflammatory cells infiltrated in the groups B, C, D. These pathological findings were more apparent in group D. Fiber connective tissue hyperplasia was attenuated, capillary hyperplasia, expansion and congestion were found in each group at week 6 postoperatively. The method of ligating abdominal aorta, iliolumbar artery and lumbar artery below renal artery for hind limb ischemia model can obtain moderate and stable ischemia conditions, maintain a long time, and is convenient to prepare.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Ischemia, Models, Animal, Arteries, Muscle Strength, Ligation, Renal Artery, Femoral Artery

CLC Number:

R394.2

Bai Chao, Yang Kun, Wang Yang, Li Xin-xi, Tian Ye, Luo Jun. Construction and evaluation of acute hind limb ischemia model in rats[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(40): 6492-6497.

Figures/Tables 3

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