Construction of rat models of failed back surgery syndrome and the possible mechanisms of Shenshu magnetic stimulation therapy

doi:10.3969/j.issn.2095-4344.2015.40.004

Abstract

Abstract:

BACKGROUND: The important reason for failed back surgery syndrome is the postoperative epidural scar adhesions, therefore, exploring the methods of preventing postoperative lumbar epidural scar adhesions has always been a hot research in spine surgery field.

OBJECTIVE: To investigate the effect of Shenshu point magnetic stimulation therapy on epidural scar adhesions in rat models of failed back surgery syndrome.

METHODS: Sixty Sprague-Dawley rat models of failed back surgery syndrome were successfully established using the method of laminectomy, and then divided into Shenshu magnetic therapy group and blank control group. Beginning from 1 week after modeling, rats in the Shenshu magnetic therapy group were subjected to bilateral Shenshu magnetic stimulation for 6 weeks, 5 days of treatment per week. Rats in the blank control group were not given any intervention. At 13 weeks after modeling, rats were harvested and the area ratio of epidural scars, range of adhesions and formation of collagen fibers were observed under light microscope. Fibroblasts were counted. Hydroxyproline content and transforming growth factor β1 expression in scar tissue were compared between these two groups.

RESULTS AND CONCLUSION: After Shenshu magnetic stimulation therapy, epidural scar tissue was reduced,a few fibroblasts infiltrated and hydroxyproline content and transforming growth factor β1 expression in the epidural scar tissue were decreased. There were very significant differences about these indices between Shenshu magnetic therapy and blank control groups (P < 0.001). These results suggest that Shenshu magnetic stimulation therapy can significantly reduce postoperative lumbar epidural scar formation, which is the possible mechanism in the treatment of failed back surgery syndrome.

Key words: Failed Back Surgery Syndrome, Postoperative Complications, Magnetic Field Therapy, Models, Animal

CLC Number:

R318

Wu Feng, Chen Xuan-huang, Huang Man-wei, Lin Hai-bin, Zheng Jin-qing. Construction of rat models of failed back surgery syndrome and the possible mechanisms of Shenshu magnetic stimulation therapy[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(40): 6413-6417.

Figures/Tables 3

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