Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (38): 6150-6154.doi: 10.3969/j.issn.2095-4344.2015.38.015

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Sealing effects of two desensitizers on the dentinal tubule: a scanning electron microscope observation 

Li Na1, Zhu Li-jiang2, Wang Xiang-lan2, Zheng Zhao-jie1   

  1. 1School of Stomatology, Weifang Medical University, Weifang 261000, Shandong Province, China; 
    2Department of Medical Care, Qingdao Stomatological Hospital, Qingdao 266071, Shandong Province, China
  • Contact: Wang Xiang-lan, Master’s supervisor, Chief physician, Department of Medical Care, Qingdao Stomatological Hospital, Qingdao 266071, Shandong Province, China
  • About author:Li Na, Studying for master’s degree, School of Stomatology, Weifang Medical University, Weifang 261000, Shandong Province, China

Abstract:

BACKGROUND: Both Ms Coat and Green OrTM desensitizers can seal dentinal tubules effectively, isolate the dentinal tubule from external stimuli and also reduce the dentinal hypersensitivity. The scholars around the world have discussed their effects from the descriptive and qualitative view.
OBJECTIVE: To quantitatively assess and compare the sealing effects of Ms Coat and Green OrTM desensitizers on dentine tubules.
METHODS: Thirty fresh human third molar samples were made into 3-mm dentin specimens that were treated with 17% EDTA for 2 minutes and randomly divided into three groups: Ms Coat group, Green OrTM group and control group with no treatment. In the former two groups, MS Coat desensitizer and Green OrTM desensitizer were applied respectively on the dentin surfaces. Scanning electron microscope was used to observe the dentin surface and longitudinal sections.
RESULTS AND CONCLUSION: (1) Surface observation: In the control group, all of the dentinal tubules were exposed; in the MS Coat group, it was observed that almost all the dentinal tubules were obliterated; in the Green OrTM group, the dentin tubules were covered with irregular crystalline substance and only a small number of exposed dentine tubules were visible but narrowed obviously. (2) Longitudinal profile observation: In the control group, the dentin tubules were strip-shaped and arranged in parallel without any blockage; in the MS coat group, the tubules were covered with dense sediments, and the dentinal tubules were basically closed; in the Green  OrTM group, there were scaly crystalline material deposited within the dentinal tubules. Dentinal diameter and number of dentinal tubules were ranked as follows: control group > Green OrTM group > MS Coat group (P < 0.05). Both Ms Coat and Green OrTM desensitizers can occlude the tubules effectively, and moreover, the MS Coat is superior to the Green OrTM.

 

中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程

Key words: Dentin Desensitizing Agents, Dentin, Pit and Fissure Sealants

CLC Number: