A static magnetic field with certain intensity can promote the secretion of nerve growth factor from Schwann cells

doi:10.3969/j.issn.2095-4344.2015.36.020

Abstract

Abstract:

BACKGROUND: There are less studies on static magnetic field, and its biological effects are still unclear.
OBJECTIVE: To investigate the static magnetic field effect on the secretion of nerve growth factor from Schwann cells.
METHODS: Schwann cells were randomly assigned into three groups: 0.05 mT sciatic magnetic field group,
0.1 mT sciatic magnetic field group and control group. At 2 days of inoculation, the cells were exposed to the sciatic magnetic field, 12 hours per day. Cells in the control group were not exposed to the sciatic magnetic field. After 6 days of exposure, RT-PCR method was used to detect nerve growth factor mRNA expression in Schwann cells, and ELISA was adopted to detect the level of nerve growth factor secreted from Schwann cells.
RESULTS AND CONCLUSION: The mRNA expression and secreted level of nerve growth factors in the culture supernatant were significantly higher in the two sciatic magnetic field groups than the control group (P < 0.05), especially in the 0.1 mT group. However, there was no difference between the 0.05 mT and 0.1 mT groups (P > 0.05). These findings indicate that the static magnetic field with certain intensity can promote the secretion of nerve growth factors from Schwann cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: chwann Cells, Nerve Growth Factor, Electromagnetic Fields

CLC Number:

R394.2

Wei Ling-yun, Wang Rui-ying, Tang Ji-cun. A static magnetic field with certain intensity can promote the secretion of nerve growth factor from Schwann cells[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(36): 5848-5852.

Figures/Tables 2

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