Effects of midbrain neural stem cells and bone marrow stromal stem cells on behaviors and brain morphology of rats with Parkinson’s disease

doi:10.3969/j.issn.2095-4344.2015.36.018

Abstract

Abstract:

BACKGROUND: For treatment of central nervous system diseases, neural stem cells (NSCs) or bone marrow stromal stem cells (BMSCs) can be transplanted into the brain, but there are less reports to compare the effects of two kinds of stem cell transplantation.

OBJECTIVE: To explore the effect of midbrain NSCs and BMSCs on the behavior and brain morphology of rats with Parkinson’s disease.

METHODS: Fifty-eight Sprague-Dawley rats were enrolled to establish Parkinson’s disease models, and then randomly divided into three groups, which were treated with 5 μL midbrain NSCs (n=20), 5 μL BMSCs (n=20) and 5 μL normal saline (n=18) via two coordinate points of the right striatum at 3 weeks after modeling, respectively. At 5 months after transplantation, the rats underwent intraperitoneal injection of apomorphine to observe behavioral changes, and then, the striatum was taken for immunohistochemistry staining.

RESULTS AND CONCLUSION: The number of rotations was reduced significantly in the BMSCs and midbrain NSCs groups at 5 months after transplantation (P < 0.05), which was significantly lower than that in the normal saline group (P < 0.05). However, there was no significant difference between the BMSCs and NSCs groups (P > 0.05). In the BMSCs group, BrdU/Nestin positive cells were seen in the brain stratium at 1 week after transplantation; BrdU/GFAP and BrdU/NSE positive cells as well as TH positive cells rather than BrdU/TH positive cells were found in the brain stratium at 1 month after transplantation; after that, the number of BrdU/Nestin positive cells was reduced gradually and disappeared ultimately, but there were still a certain number of BrdU/GFAP and BrdU/NSE positive cells, especially the former ones. Meanwhile, the NSCs group also had a similar situation, but no double-labeled cells were in the normal saline group. These findings indicate that midbrain NSCs and BMSCs transplantation can both improve the behavior of Parkinson’s disease rats, and differentiate into neurons, astrocytes and dopaminergic neurons.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Parkinson Disease, Neural Stem Cells, Bone Marrow, Mesenchymal Stem Cells, Neurobehavioral Manifestations

CLC Number:

R394.2

Liang Xin-ming, Fu Guo-hui, Zhang Bao-chao. Effects of midbrain neural stem cells and bone marrow stromal stem cells on behaviors and brain morphology of rats with Parkinson’s disease [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(36): 5838-5842.

Figures/Tables 2

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