METHODS: Adipose-derived mesenchymal stem cells were isolated from the subcutaneous fat of hepatitis B virus infection patients by collagenase digestion and adherent method. Growth curve of adipose-derived mesenchymal stem cells were detected by MTT method and cell phenotypes were detected by flow cytometry. The adipogenic and osteogenic differentiation potential of adipose-derived mesenchymal stem cells were detected in vitro.

RESULTS AND CONCLUSION: (1) Adipose-derived mesenchymal stem cells from 10 patients with hepatitis B virus infection were all isolated and cultured successfully. The primary passage time of adipose-derived mesenchymal stem cells was (8.3±1.2) days. The growth curve of cells was “S” shaped. Cells came into a logarithmic phase at days 3-4, and came into platform at day 7. (3) Passage 3 adipose-derived mesenchymal stem cells highly expressed CD29, CD166, HLA-ABC and CD44, but did not express or lowly expressed CD34 and HLA-DR. (3) Adipose-derived mesenchymal stem cells differentiated into adipocytes after adipogenic induction, and differentiated cells were positive for oil red O staining; after osteogenic induction, adipose-derived mesenchymal stem cells differentiated into osteoblasts that were positive for alkaline phosphatase staining. These findings indicate that the collagenase digestion and adherent method can be used to effectively isolate adipose-derived mesenchymal stem cells from patients with hepatitis B virus infection, and the cell proliferation is rapid so that a large number of cells can be obtained in the short term.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程