SPIO and DAPI double labeled bone marrow mesenchymal stem cells of macaques: effects on cell viability and proliferation

doi:10.3969/j.issn.2095-4344.2015.36.001

Abstract

Abstract:

BACKGROUND: Traditional cell transplantation tracer methods require histological analysis and identification in vitro, which limits the clinical application of stem cell transplantation. So it is urgent to establish an in vivo noninvasive and repeatable tracer method.
OBJECTIVE: To observe the effect of SPIO and DAPI double labeling on survival and proliferation of bone marrow mesenchymal stem cells from macaques.
METHODS: Bone marrow mesenchymal stem cells were derived from bone marrow aspirates of healthy macaques using whole bone marrow adherence method. Then, the cells were identified using flow cytometry detection. Bone marrow mesenchymal stem cells were labeled using SPIO and DAPI. Fluorescent microscope was used to detect DAPI positive rate, and Prussian blue staining and transmission electron microscope were employed to measure SPIO positive rate. MTT assay was used to detect cell viability and proliferation.
RESULTS AND CONCLUSION: Bone marrow mesenchymal stem cells were successfully isolated from healthy macaques using the whole bone marrow adherence method, and the cell purity was up to 95.1%. SPIO and DAPI
were both successful to label the bone marrow mesenchymal stem cells with a positive rate of 95%-98%, but had no influence on cell viability and proliferation.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cells, Magnetite Nanoparticles

CLC Number:

R394.2

Song Qiao-qiao, Zhou Hui-liang, Pan Xing-hua. SPIO and DAPI double labeled bone marrow mesenchymal stem cells of macaques: effects on cell viability and proliferation [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(36): 5741-5745.

Figures/Tables 3

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