Effects of medicated serum containing Duhuojisheng Decoction on the expression of cytochrome C, proCaspase-9 and proCaspase-3 in degenerative chondrocytes

doi:10.3969/j.issn.2095-4344.2015.33.005

Abstract

Abstract:

BACKGROUND: Previous studies have shown that apoptosis, a central feature of articular chondrocytes, plays a dominant role in cartilage damage, which is one of the pathological factors of articular cartilage degeneration.
OBJECTIVE: To observe the effects of meditcated serum containing Duhuojisheng Decoction on the expression of cytochrome C, proCaspase-9 and proCaspase-3 in rat degenerative chondrocytes in vitro and to investigate the possible molecular biological mechanism of Duhuojisheng Decoction in the treatment of knee osteoarthritis.
METHODS: A cultivation system of degenerative chondrocytes in vitro was established. After treatment with meditcated serum containing Duhuojisheng Decoction or blank serum for 24 and 48 hours, the protein expression of cytochrome C, proCaspase-9 and proCaspase-3 was measured by western blot assay.
RESULTS AND CONCLUSION: In the cytoplasm, the release of cytochrome C was reduced gradually in both groups in a time-dependent manner, and the release amount of cytochrome C was significantly lower in the medicated serum group than the blank serum group (P < 0.05). In mitochondria, cytochrome C leakage was gradually decreased in both groups, and it was decreased significantly in the medicated serum group compared with the blank serum group (P < 0.05). The protein expression of proCaspase-9 and proCaspase-3 was gradually increased in both groups, especially in the medicated serum group; the medicated serum containing Duhuojisheng Decoction could promote the protein expression of proCaspase-9 and proCaspase-3 in a time-dependent manner, and there was a significant difference at 24 and 48 hours (P < 0.01). These findings indicate that the medicated serum containing Duhuojisheng Decoction can inhibit the apoptosis of osteoarthritis chondrocytes through inhibiting the release of cytochrome C and the activation of Caspase-9 and Caspase-3.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Osteoarthritis, Knee Joint, Drugs, Chinese Herbal, Cytochromes c

CLC Number:

R318

Wang Wu-lian, Lin Yu, Zhang Yi-yuan, Xiao Li-li, Feng Er-you. Effects of medicated serum containing Duhuojisheng Decoction on the expression of cytochrome C, proCaspase-9 and proCaspase-3 in degenerative chondrocytes[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(33): 5269-5273.

Figures/Tables 2

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