Stomach cancer stem cells: isolation, identification and characteristics

doi:10.3969/j.issn.2095-4344.2015.32.019

Abstract

Abstract:

BACKGROUND: With the in-depth study of cancer stem cells, increasing evidence has shown that cancer stem cells are the reason for cancer metastasis and recurrence, and therefore it is of great significance to isolate and identify cancer stem cells that can elucidate the pathogenesis of cancer and development of anticancer drugs.
OBJECTIVE: To isolate and culture gastric cancer stem cells and then to detect its biological characteristics.
METHODS: Cancer tissues from 16 patients with gastric cancer were cultured using adherent method and enzymic digestion methods to isolate gastric cancer stem cells. The cell morphology was observed under inverted microscope to drawn out growth curves. Osteogenic and adipogenic ability of cells were also detected.
RESULTS AND CONCLUSION: Gastric cancer stem cells were isolated successfully by both two methods. Under the microscope, the cells were fusiform- or polygon-shaped. At confluence, the cells grew in a vortex or radial manner. The cell growth curve showed that 1-3 days were latent period, 4-9 days were logarithmic growth phase, and over 10 days were growth plateau phase. Flow cytometry results showed that passage 3 gastric cancer stem cells highly expressed CD90, CD29, CD44, but lowly expressed CD34, CD45 and HLA-DR. After osteogenic induction, calcified nodules were clearly visible in the gastric cancer stem cells at passage 3; after adipogenic induction, bright and tiny fat droplets were seen in the cytoplasm. These findings indicate that cancer stem cells exist in the gastric cancer tissues, and have similar morphology, biological property and multipotent differentiation ability to normal cells, which are probably involved in gastric cancer occurrence and development.

Key words: Stomach Neoplasms, Tumor Cells, Cultured, Neoplastic Stem Cells

CLC Number:

R394.2

Li Yang, Zhao Yong-fu. Stomach cancer stem cells: isolation, identification and characteristics[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(32): 5188-5191.

Figures/Tables 2

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