Correlation between functional status of bone marrow mesenchymal stem cells and disease progression in osteoarthritis patients

doi:10.3969/j.issn.2095-4344.2015.32.009

Abstract

Abstract:

BACKGROUND: There is a certain relationship between bone marrow mesenchymal stem cells and articular cartilage, and its cellular functional status is one of the causes of osteoarthritis.
OBJECTIVE: To explore the correlation between functional status of bone marrow mesenchymal stem cells and disease progression in patients with osteoarthritis.
METHODS: The clinical data of 45 patients were collected, including 15 cases undergoing arthroscopic knee cruciate ligament reconstruction (control group), 15 cases of mild osteoarthritis (mild osteoarthritis group), 15 cases of severe osteoarthritis (severe osteoarthritis group). Bone marrow mesenchymal stem cells from three groups were cultured in vitro to observe the morphological changes. Proliferative and differentiation ability was detected and compared between the three groups.
RESULTS AND CONCLUSION: At 24 hours of primary culture, the bone marrow mesenchymal stem cells were arranged in regular pattern and spindle-shaped in the three groups with no morphological differences. At 14 days of chondrogenic induction, toluidine blue staining was positive for bone marrow mesenchymal stem cells, but there was still no difference in the chondrogenic ability in the three groups. Cell counting kit-8 results showed that the proliferative ability of cells was strongest in the control group and weakest in the severe osteoarthritis group. These findings indicate that there is a certain correlation between the functional status of bone marrow mesenchymal stem cells and the progress of the disease.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Osteoarthritis, Bone Marrow, Mesenchymal Stem Cells

CLC Number:

R394.2

Liu Jian. Correlation between functional status of bone marrow mesenchymal stem cells and disease progression in osteoarthritis patients [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(32): 5129-5133.

Figures/Tables 2

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