Hydrogen-rich water inhibits mitochondrial oxidative stress and inflammation in the skeletal muscle after eccentric exercise

doi:10.3969/j.issn.2095-4344.2015.29.018

Abstract

Abstract:

BACKGROUND: It is unclear whether hydrogen-rich water can be used to protect skeletal muscle injury induced by eccentric exercise, as well as the relative mechanism.
OBJECTIVE: To observe the effect of hydrogen-rich water on the mitochondrial oxidative stress and inflammation in rat skeletal muscle after eccentric exercise, and to investigate the relative signaling pathway of hydrogen-rich water.
METHODS: Forty Sprague Dawley rats were randomly divided into four groups: control group, eccentric exercise group, eccentric exercise+saline group, and eccentric exercise+hydrogen-rich water group. Rats in three eccentric exercise groups were exercised on a motor-driven rodent treadmill at a speed of 16-18 m/min and a slope of -16° for 90 minutes per day. Rats in the eccentric exercise+hydrogen-rich water group were subjected to intraperitoneal injection of hydrogen-rich water (10 mL/kg) immediately after exercise; and rats in the eccentric exercise+saline group were administrated with normal saline after exercise. All the interventions lasted for 5 days.
RESULTS AND CONCLUSION: Hydrogen-rich water intervention after eccentric exercise could markedly enhance the mitochondrial Sirtuin-3 expression, improve the mitochondrial membrane potential and activity of manganese superoxide dismutase, down-regulate the mitochondrial reactive oxygen species generation and
mitochondrial DNA oxidative damage, thus inhibiting inflammatory cytokines expression, such as NLRP3 and interleukin-1β. The results indicated that hydrogen-rich saline could directly scavenge reactive oxygen species. In addition, hydrogen-rich water could improve mitochondrial energy metabolism and antioxidant capacity through up-regulation of Sirtuin-3, which in turn inhibits eccentric exercise-induced mitochondrial oxidative stress and secondary inflammation in the skeletal muscle.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Hydrogen, Hyperkinesis, Inflammation, Mitochondria

CLC Number:

R318

Wang Lei, Liu Zi-quan, Hou Yi-ling, Ge Yao-jun. Hydrogen-rich water inhibits mitochondrial oxidative stress and inflammation in the skeletal muscle after eccentric exercise [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(29): 4682-4687.

Figures/Tables 3

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