Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (28): 4439-4444.doi: 10.3969/j.issn.2095-4344.2015.28.003

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Effect of Longbie Capsule on proliferation of bone marrow mesenchymal stem cells

Pan Jian-ke1, Guo Bai-ming1, Liu Jun2, Zhang Xian3, Xie Hui1, Guo Da2   

  1. 1Second School of Clinic Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong Province, China; 
    2Department of Orthopedics, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510120, Guangdong Province, China; 
    3Centre Laboratory, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510120, Guangdong Province, China
  • Online:2015-07-02 Published:2015-07-02
  • Contact: Liu Jun, Chief physician, Doctoral supervisor, Department of Orthopedics, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510120, Guangdong Province, China
  • About author:Pan Jian-ke, Studying for doctorate, Second School of Clinic Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81473698, 81273781; the Specialized Research Fund for the Doctoral Program of Higher Education, Ministry of Education, No. 20124425110004; the Science and Technology Plan of Guangdong Province, No. 2011B031700027; the Project of Guangdong Finance Department, No. [2014]157; the Specialized Fund for Chinese Medicine Research in Guangdong Provincial Hospital of Chinese Medicine, No. YK2013B2N1

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Abstract:

BACKGROUND:Longbie Capsule has satisfactory outcomes in the treatment of osteoarthritis, but its mechanism is still unclear.

OBJECTIVE: To study the effect of Longbie Capsule on proliferation of bone marrow mesenchymal stem cells.
METHODS: The bone marrow mesenchymal stem cells from SD rats were separated and expanded by whole adherence culture, then subcultured and confirmed by morphological observation and flow cytometry. Passage 4 cells were cultured in complete media containing 5 g/L, 1 g/L, 250 mg/L, 50 mg/L, 10 mg/L Longbie Capsule, respectively, for 24 hours. Then, MTT assay was used to detect cell viability.
RESULTS AND CONCLUSION: The primary cells were adherent cells characterized by irregular shape, passage 2 cells were typically fibrous-shaped, passage 3 cells grew in long fibrous and swirl-type shape. Passage 4 cells were strongly positive for CD29 and CD90, positive for CD44, and negative for CD34 and CD45. 5 g/L and 1 g/L Longbie Capsule promoted the proliferation of bone marrow mesenchymal stem cells. These findings indicate that Longbie Capsule may promote the proliferation and differentiation of bone marrow mesenchymal stem cells, thereby playing a therapeutic effect on osteoarthritis.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Osteoarthritis, Bone Marrow, Mesenchymal Stem Cells

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