Recombinant human bone morphogenetic protein 2 composite bone in the lumbar spine fusion

doi:10.3969/j.issn.2095-4344.2015.25.005

Abstract

Abstract:

BACKGROUND: Many studies have confirmed that recombinant human bone morphogenetic protein 2 plays a very important role in bone formation and fracture healing, but recombinant human bone morphogenetic protein 2 alone implanted is prone to diffusion and degradation, which is unable to play a persistent role in new bone formation.
OBJECTIVE: To explore the effect of recombinant human bone morphogenetic protein 2 composite bone in the rabbit lumbar fusion.
METHODS: Thirty New Zealand white rabbits were selected to make posterior lumbar intertransverse fusion models, and then were randomly divided into three groups, in which, L5-6 intertransverse implantation of autologous iliac bone, allogeneic bone and recombinant human bone morphogenetic protein 2 composite bone
(recombinant human bone morphogenetic protein 2 and allogeneic bone complex) was done respectively. At 6 weeks after implantation, gross observation, X-ray examination and histological observation were performed.
RESULTS AND CONCLUSION: Fusion rate and percentage of new bone area were higher in the composite bone group than the autologous iliac bone and allogeneic bone groups (P < 0.05); the tensile strength was lower in the allogeneic bone group than the other two groups (P < 0.05), but there was no difference between these two groups except the allogeneic bone group. X-ray films showed callus formation in the implanted region of the three groups. In the autologous iliac bone group, a large amount of cartilage tissues formed along with a small amount of bone trabeculae and a certain amount of woven bones. In the allogeneic bone group, the implant was covered with a large amount of fibrous tissues, bone island was seen and there was also a small amount of bone trabeculae and cartilage tissues. In the composite bone group, a great amount of bone trabeculae and cartilage tissues were visible to form woven bone and cortical bone. These findings indicate that the recombinant human bone morphogenetic protein 2 composite bone can obtain good effect in the rabbit lumbar fusion.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Bone Morphogenetic Proteins, Bone Transplantation, Spinal Fusion

CLC Number:

R318

Wang Zhan, Zhang Jun, Wang Deng-feng, Shao Yu-xiong, Wei Wei, Zhu Yang-jun, Wang Wei. Recombinant human bone morphogenetic protein 2 composite bone in the lumbar spine fusion [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(25): 3957-3961.

Figures/Tables 2

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