Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (23): 3768-3772.doi: 10.3969/j.issn.2095-4344.2015.23.027

Previous Articles    

Fate of reprogramming cells

Ruan Guang-ping, Yao Xiang, Liu Ju-fen, Hu Yuan-yuan, Wang Jin-xiang, He Jie, Zhao Jing, Pan Xing-hua   

  1. The Cell Biological Therapy Center, Kunming General Hospital of Chengdu Military Command, State & Regions Integrated Engineering Laboratory of Stem Cells and Immune Cells Biomedical Techniques, Yunnan Provincial Key Laboratory
  • Online:2015-06-04 Published:2015-06-04
  • Contact: Pan Xing-hua, M.D., Chief physician, the Cell Biological Therapy Center, Kunming General Hospital of Chengdu Military Command, State & Regions Integrated Engineering Laboratory of Stem Cells and Immune Cells Biomedical Techniques, Yunnan Provincial Key Laboratory of Cell Therapy Technology Transfer Medicine, Kunming 650032, Yunnan Province, China
  • About author:Ruan Guang-ping, M.D., Associate chief physician, the Cell Biological Therapy Center, Kunming General Hospital of Chengdu Military Command, State & Regions Integrated Engineering Laboratory of Stem Cells and Immune Cells Biomedical Techniques, Yunnan Provincial Key Laboratory of Cell Therapy Technology Transfer Medicine, Kunming 650032, Yunnan Province, China
  • Supported by:

    the National Support Program of China, No. 2014BAI01B01; Yunnan Provincial Science and Technology Project, No. 2013CA005

PDF

248

Abstract:

BACKGROUND: Somatic cell reprogramming technology, also known as recombinant technology, has completed differentiated somatic cells back to the original totipotent or pluripotent state, and can be re-differentiated into cells different from original ones. Re-programming techniques are able to harvest specifically induced pluripotent stem cells and disease-specific induced pluripotent stem cells from patients, which can significantly reduce the immune rejection.
OBJECTIVE: To explore the method from direct reprogramming to specific cell lines and to conclude the
molecular mechanisms underlying reprogramming.
METHODS: A computer-based search of VIP, Wanfang, CNKI, PubMed and Springer databases was performed for articles related to cell reprogramming techniques published from January 1958 to April 2015 using the keywords of “reprogramming” in Chinese and English, respectively. After elimination of unrelated and repetitive studies, 40 articles were retained for further analysis.
RESULTS AND CONCLUSION: Current reprogramming steps are inefficient that in the specific group only a relatively small number of cells can be reprogrammed, and the extent and integrity of reprogramming are questionable. Direct reprogramming of adult cells and specific cell lines that are changed to another line is always a difficulty in developmental biology. Recent studies have proved that differentiated cells can forcibly express specific transcription factor to improve cell differentiation. This discovery is a great progress in regenerative medicine that cell replacement therapy can be provided for renewable disorders. At present, the basic molecular mechanisms require further clarification, and there are many problems to be solved before the direct reprogramming is used clinically.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Nuclear Reprogramming, Transcription Factor, Induced Pluripotent Stem Cells

CLC Number: