Isoflurane inhibits proliferation and differentiation of neural stem cells in the dentate gyrus

doi:10.3969/j.issn.2095-4344.2015.19.017

Abstract

Abstract:

BACKGROUND: Isoflurane is gradually popularized in pediatric surgery due to its rapid onset, rapid recovery, and no savings, but its security needs to be further studied.
OBJECTIVE: To explore the effect of isoflurane anesthesia on the proliferation and neuronal differentiation of neural stem cells in the dentate gyrus of neonatal rats.
METHODS: Rats were randomly divided into isoflurane group and control group, respectively treated with isoflurane inhalation and air inhalation. 5-Bromodeoxyuridine ball glycosides (BrdU) was intraperitoneally injected before drug administration and after drug withdrawal, and 24 hours after secondary injection, the rats were executed to take brain tissues for detection of BrdU expression and brain neurogenic differentiation factor expression.
RESULTS AND CONCLUSION: After cessation of anaesthesia processing for the detection of blood glucose and arterial blood gas, a mild rise in PaCO2 was found in the isoflurane group and pH showed a slight drop, but PaO2, BE, SaO2 and blood glucose levels were not changed. Compared with the control group, the isoflurane group did not appear with obvious hypoxia performances after isoflurane anesthesia, including cyanosis and respiratory depression. Rat neural stem cells of the dentate gyrus were mostly distributed in the gate area, and compared with the control group, the number of BrdU-positive cells was less in the isoflurane group (P < 0.05). A large number of new cells in the dentate gyrus expressed NeuroD, and the number of NeuroD+/BrdU+ cells in the gate area was significantly higher than that in the lower region of granule cells. Moreover, the number of
NeuroD+/BrdU+ cells in the isoflurane group was significantly higher than that in the control group (P < 0.05). These findings suggest that isoflurane anesthesia can influence the proliferation and neuronal differentiation of neural stem cells in the dentate gyrus of neonatal rats, which inhibits cell proliferation and promotes neuronal differentiation.

Key words: Isoflurane, Dentate Gyrus, Behavior, Animal, Therapeutic Uses, Anesthesia

CLC Number:

R394.2

Lu Cheng-kang. Isoflurane inhibits proliferation and differentiation of neural stem cells in the dentate gyrus[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(19): 3049-3053.

Figures/Tables 2

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