Expression of hepatic energy proteins following reduced-size liver transplantation in rats

doi:10.3969/j.issn.2095-4344.2015.18.015

Abstract

Abstract:

BACKGROUND: At present, the proteome is a mature technology that has been applied in basic research fields related to liver transplantation. But, it has been not reported in research related to reduced-size liver transplantation.
OBJECTIVE: To explore the expression of differential proteins related to hepatic energy metabolism following reduce-size liver transplantation in rats by using by proteomic technology.
METHODS: The improved model of reduced-size liver transplantation was used in this experiment. The donor was health female Lewis rats and the recipient was male Wistar rats for liver transplantation. The difference between the donor and the recipient was about 20 g. The weight of donor liver/the weight of recipient donor was approximately equal to 50%. The donor liver tissue was harvested and trimmed to the required size. The portal vein and infrahepatic vena cava were cannulated, and the biliary tract was implanted into the donor bile duct for
transplantation. Then the donor was transplanted into the recipient after the removal of original liver tissue. Hepatic specimens were harvested by 1, 3 and 7 days after reduced-size liver transplantation. Then, the harvested specimens were compared with the normal donor and recipient liver tissue that were previously harvested and frozen, to generate two-dimensional gel electrophoresis profile using proteome technology. Then tandem mass spectrometry and databases analysis were performed after two-dimensional electrophoresis for identifying differential protein stains.
RESULTS AND CONCLUSION: In this experiment, 72 differential protein stains with over lo-fold changes were selected. After identification, 32 proteins showed clear functions, and among them three differential proteins (ATP synthase beta subunit, electron-transferring flavoprotein beta peptide and proton-transferring ATP synthase) were involved in the process of cell energy metabolism. The proteins were distributed on 1 and 7 days after reduce-size liver transplantation, accounting for 6%.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: Liver Transplantation, Proteomics, ATP Synthase Complex, Energy Metabolism

CLC Number:

R318

Liu Jing, Li Li, Ran Jiang-hua, Zhang Sheng-ning, Li Lai-bang, Zhang Xi-bing, Gao Yang, Chen Yi-ming. Expression of hepatic energy proteins following reduced-size liver transplantation in rats[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(18): 2874-2878.

Figures/Tables 3

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