Transfection of double gene co-expressing adenovirus vector into arthritis rats

doi:10.3969/j.issn.2095-4344.2015.18.007

Abstract

Abstract:

BACKGROUND: Abnormal activation of lymphocytes and nuclear factor κB-dependent non-specific inflammation are two major manifestations of joint damage in rheumatoid arthritis. Co-stimulatory signal CD40/CD40L is the dominant co-stimulatory factor in the recognition and activation of T cells. IκBα effectively inhibits nuclear factor κB pathway, prevent the inflammation in the central link, and suppress the damage caused by inflammatory factor in the synovial tissue.
OBJECTIVE: To investigate the therapeutic effect of double gene co-expressing adenovirus vector on arthritis based on an arthritis model rat transfected by CD40LIg-IRES2-IκBα co-expressing adenovirus vector.
METHODS: The pAdCD40LIg-IRES2-IκBα co-expressing adenovirus vector was established. Arthritic model was established through multi-subcutaneous injections of complete Freund's adjuvant of type collagen II (1 g/L) into Wistar rats. Then 20 arthritic rats were divided into two groups: untreated group and transfection group, receiving an injection of saline and pAdCD40LIg-IRES2-IκBα adenovirus vector to distal joint cavity of limbs, respectively.
RESULTS AND CONCLUSION: At 14 days post-transfection, compared with the untreated group, the mean arthritis index score, the CD40L expression of lymphocytes in synovial fluid, the nuclear factor-κB p65 expression in synovial tissue, and levels of interleukin-2, interleukin-6, tumor necrosis factor-α, matrix metalloproteinase-3 and matrix metalloproteinase-9 in synovial fluid of rats in transfection group were significantly lower than those in
untreated group. Focal transfection of the CD40LIg-IκBα co-expression adenovirus vector can effectively inhibit arthritic symptoms, and reduce the expressions of inflammatory cytokine in synovial fluid and inflammatory molecule in synovial tissue of arthritic rats, which shows good therapeutic effect.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: Arthritis, Adenoviridae Infections, Lymphocytes, T-Lymphocytes

CLC Number:

R318

Fan Ping, He Lan, Hao Zhi-ming1, Pu Dan, Lv Xiao-hong, Sun Yi-ning, Hu Nan, Wang Yan-hua,Ding Xiao-ming, Li Yang, Xue Wu-jun. Transfection of double gene co-expressing adenovirus vector into arthritis rats[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(18): 2825-2830.

Figures/Tables 4

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