Preparing acellular nerve allografts by combined freeze-thaw and chemical methods

doi:10.3969/j.issn.2095-4344.2015.12.021

Abstract

Abstract:

BACKGROUND: Host immune rejection is the main problem for nerve allograft in the repair of nerve defects. Therefore, how to avoid and minimize the immune rejection is the key to the success of nerve allografting.
OBJECTIVE: To develop a new nerve pretreatment method by which Schwann cells and myelin can be removed from the peripheral nerve of dogs while the basilar membrane can be reserved integrally in order to obtain acellular nerve allografts.
METHODS: Bilateral sciatic nerves from healthy adult dogs were taken and pretreated with the combined freeze-thaw and chemical methods followed by microscopic observation of ultrastructural features, histological staining and western blot analysis of its ingredients.
RESULTS AND CONCLUSION: Pretreated acellular nerves with good ductility and excellent epineurium toughness were empty basal lamina tubes with no Schwann cells, myelin and fragments that were all removed thoroughly, but the basilar membrane was fully retained. These findings indicate that the optimized combination of freeze-thaw and chemical methods can efficiently clear Schwann cells and myelin which are the major antigenic components in the peripheral nerve, while preserve the basilar membrane to promote nerve regeneration. Therefore, this method can be an ideal method for preparation of tissue engineered nerves.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: Peripheral Nerves, Transplantation, Homologous, Schwann Cells

CLC Number:

R318

Guan Shu-jun, Wang Wei, Li Yan. Preparing acellular nerve allografts by combined freeze-thaw and chemical methods[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(12): 1914-1918.

Figures/Tables 5

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