A new method to isolate mesenchymal stem cells from human placenta

doi:10.3969/j.issn.2095-4344.2015.10.024

Abstract

Abstract:

BACKGROUND: Placenta is a valuable source of mesenchymal stem cells for stem cell therapy and future application in the field of regenerative medicine. However, conventional methods cannot acquire a large amount of purified human placenta-derived mesenchymal stem cells. Here, we present a new method for isolating human placenta-derived mesenchymal stem cells suitable for banking strategies and for future clinical applications.

OBJECTIVE: To analyze the biological characteristics of human placenta-derived mesenchymal stem cells cultured by tissue dissociating and collagenase digestion.

METHODS: Human placenta-derived mesenchymal stem cells were obtained from human placenta by tissue dissociating and collagenase digestion method. Immunophenotype was analyzed by flow cytometry. Growth curve was determined by MTT assay, and differentiation ability was evaluated by in vitro adipogenic, osteogenic and chondrogenic induction as well.

RESULTS AND CONCLUSION: Human placenta-derived mesenchymal stem cells could be passaged stably in vitro. Furthermore, the cells expressed CD73, CD90, CD105, but were negative for the markers of CD11b, CD19, CD34, CD45, and HLA-DR. Human placenta-derived mesenchymal stem cells proliferated actively and began to grow logarithmically at days 3-5 followed by a plateau period at day 6. In addition, the isolated cells could be induced into adipocytes, osteocytes, chondrocytes in vitro. In a word, the results of this study demonstrated that the tissue dissociating and collagenase digestion method is an efficient method for obtaining a large amount of human placenta-derived mesenchymal stem cells that can be stably cultured in vitro and have strong proliferative ability.

1库：

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Placenta, Mesenchymal Stem Cells, Cell Culture Techniques

CLC Number:

R394.2

Liu Yang, Li Yan-qi, Wang Hong-yi, Wu Xiao-bing, Jing Yong-guang, Xu Xiao, Yao Yao, Zhang Yu, Chu-Tse Wu, Jin Ji-de. A new method to isolate mesenchymal stem cells from human placenta[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(10): 1608-1612.

Figures/Tables 4

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