Chondrogenic differentiation of mesenchymal stem cells on matrigel/chitosan scaffolds

doi:10.3969/j.issn.2095-4344.2015.10.006

Abstract

Abstract:

BACKGROUND: Previous studies have found that the basement membrane matrix can induced the chondrogenic differentiation of bone marrow mesenchymal stem cells, but there is a wide gap between its mechanical properties and practical application, and further research is needed.

OBJECTIVE: To prepare a suitable matrigel/chitosan scaffold that has appropriate mechanical properties and remarkable bioactivity for cartilage repair.

METHODS: We selected genipin as cross-linking agent, and mixed Matrigel with cross-linked chitosan at different ratios (2:1, 1:1, 1:3). Then rat bone marrow mesenchymal stem cells were seeded on different scaffolds and cultured for 14 days. The mechanical properties of materials were measured by DMA. Cell counting kit-8, FDA staining, ELISA kits and Alcian blue staining were used to measure the bioactivity of materials.

RESULTS AND CONCLUSION: The storage modulus of scaffolds was raised from 0.48 kPa to 1.78 kPa with increase ratio of chitosan. Cells spread well in the early period on all scaffolds, and then the cells on the chitosan scaffold showed reduced chondrogenic activity, but cells on the scaffolds with matrigel could maintain chondrogenic differentiation. The matrigel/chitosan scaffold at a ratio of 1:1 had appropriate mechanical properties and higher levels of collagen II and collagen X at 14 days. The prepared matrigel/chitosan scaffold with decent mechanical performance can promote the differentiation of bone marrow mesenchymal stem cells into chondrogenic lineages, which can be used in cartilage tissue engineering.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Bone Marrow, Mesenchymal Stem Cells, Basilar Membrane, Chitosan

CLC Number:

R394.2

Dai Yun, He Jing, Wu Fang . Chondrogenic differentiation of mesenchymal stem cells on matrigel/chitosan scaffolds[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(10): 1506-1510.

Figures/Tables 5

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